NDLI: Induced fit of a peptide loop of methionyl-tRNA formyltransferase triggered by the initiator tRNA substrate.

Please wait, while we are loading the content...

Year: 2017 Volume: 114

Year: 2016 Volume: 113

Year: 2015 Volume: 112

Year: 2014 Volume: 111

Year: 2013 Volume: 110

Year: 2012 Volume: 109

Year: 2011 Volume: 108

Year: 2010 Volume: 107

Year: 2009 Volume: 106

Year: 2008 Volume: 105

Year: 2007 Volume: 104

Year: 2006 Volume: 103

Year: 2005 Volume: 102

Year: 2004 Volume: 101

Year: 2003 Volume: 100

Year: 2002 Volume: 99

Year: 2001 Volume: 98

Year: 2000 Volume: 97

Year: 1999 Volume: 96

Year: 1999 Volume: 96 Issue: 26

Year: 1999 Volume: 96 Issue: 25

Year: 1999 Volume: 96 Issue: 24

Year: 1999 Volume: 96 Issue: 23

Year: 1999 Volume: 96 Issue: 22

Year: 1999 Volume: 96 Issue: 21

Year: 1999 Volume: 96 Issue: 20

Year: 1999 Volume: 96 Issue: 19

Year: 1999 Volume: 96 Issue: 18

Year: 1999 Volume: 96 Issue: 17

Year: 1999 Volume: 96 Issue: 16

Year: 1999 Volume: 96 Issue: 15

Year: 1999 Volume: 96 Issue: 14

Year: 1999 Volume: 96 Issue: 13

Year: 1999 Volume: 96 Issue: 12

Year: 1999 Volume: 96 Issue: 11

Year: 1999 Volume: 96 Issue: 10

Year: 1999 Volume: 96 Issue: 9

Year: 1999 Volume: 96 Issue: 8

Year: 1999 Volume: 96 Issue: 7

Year: 1999 Volume: 96 Issue: 6

Year: 1999 Volume: 96 Issue: 5

Year: 1999 Volume: 96 Issue: 4

Year: 1999 Volume: 96 Issue: 3

Tet B or not tet B: advances in tetracycline-inducible gene expression.

Disease transmission dynamics and the evolution of antibiotic resistance in hospitals and communal settings.

Xenotropism: the elusive viral receptor finally uncovered.

Niemann-Pick C1 protein: Obligatory roles for N-terminal domains and lysosomal targeting in cholesterol mobilization

Origins of DNA-binding specificity: role of protein contacts with the DNA backbone.

Trimeric domain-swapped barnase.

Three-dimensional structure of phosphoenolpyruvate carboxylase: a proposed mechanism for allosteric inhibition.

Xenopus laevis sperm receptor gp69/64 glycoprotein is a homolog of the mammalian sperm receptor ZP2.

Levels of major selenoproteins in T cells decrease during HIV infection and low molecular mass selenium compounds increase.

Structure of the ternary complex of human 17beta-hydroxysteroid dehydrogenase type 1 with 3-hydroxyestra-1,3,5,7-tetraen-17-one (equilin) and NADP+.

Mitochondrial disease in superoxide dismutase 2 mutant mice.

U1 small nuclear RNA and spliceosomal introns in Euglena gracilis.

The specificity of interaction of archaeal transducers with their cognate sensory rhodopsins is determined by their transmembrane helices.

TOXCAT: a measure of transmembrane helix association in a biological membrane.

The C-terminal domain of the regulatory protein NOVH is sufficient to promote interaction with fibulin 1C: a clue for a role of NOVH in cell-adhesion signaling.

Induced fit of a peptide loop of methionyl-tRNA formyltransferase triggered by the initiator tRNA substrate.

Similarity in the catalysis of DNA breakage and rejoining by type IA and IIA DNA topoisomerases.

The minimal gene set member msrA, encoding peptide methionine sulfoxide reductase, is a virulence determinant of the plant pathogen Erwinia chrysanthemi.

Single-molecule fluorescence spectroscopy of enzyme conformational dynamics and cleavage mechanism.

Enhanced rate of intramolecular electron transfer in an engineered purple CuA azurin.

Alpha-helix nucleation by a calcium-binding peptide loop.

SEM1, a homologue of the split hand/split foot malformation candidate gene Dss1, regulates exocytosis and pseudohyphal differentiation in yeast

Expression cloning of LDLB, a gene essential for normal Golgi function and assembly of the ldlCp complex.

Dimensional and mechanical dynamics of active and stable edges in motile fibroblasts investigated by using atomic force microscopy.

Cloning and characterization of a cell surface receptor for xenotropic and polytropic murine leukemia viruses.

The nuclear matrix prepared by amine modification.

Model of maltose-binding protein/chemoreceptor complex supports intrasubunit signaling mechanism.

Multiple and diverse forms of regulated exocytosis in wild-type and defective PC12 cells

FOG-2: A novel GATA-family cofactor related to multitype zinc-finger proteins Friend of GATA-1 and U-shaped.

Molecular cloning of FOG-2: a modulator of transcription factor GATA-4 in cardiomyocytes.

Metamorphosis is inhibited in transgenic Xenopus laevis tadpoles that overexpress type III deiodinase.

sqv mutants of Caenorhabditis elegans are defective in vulval epithelial invagination.

Three proteins involved in Caenorhabditis elegans vulval invagination are similar to components of a glycosylation pathway.

Perception of solar UVB radiation by phytophagous insects: behavioral responses and ecosystem implications.

Interspecific and intraspecific competition as causes of direct and delayed density dependence in a fluctuating vole population.

Species realities and numbers in sexual vertebrates: perspectives from an asexually transmitted genome.

Ectopic expression of E47 or E12 promotes the death of E2A-deficient lymphomas.

Inhibition of cyclin-dependent kinase 2 by p21 is necessary for retinoblastoma protein-mediated G1 arrest after gamma-irradiation.

Progressive juvenile-onset punctate cataracts caused by mutation of the gammaD-crystallin gene.

Generation of conditional mutants in higher eukaryotes by switching between the expression of two genes.

CD48-deficient mice have a pronounced defect in CD4(+) T cell activation.

T cell antigen receptor-mediated activation of the Ras/mitogen-activated protein kinase pathway controls interleukin 4 receptor function and type-2 helper T cell differentiation.

Lack of infection in HIV-exposed individuals is associated with a strong CD8(+) cell noncytotoxic anti-HIV response.

Distinct dendritic cell subsets differentially regulate the class of immune response in vivo.

Identification of a cell protein (FIP-3) as a modulator of NF-kappaB activity and as a target of an adenovirus inhibitor of tumor necrosis factor alpha-induced apoptosis.

The stretch-activation response may be critical to the proper functioning of the mammalian heart.

Defective collagen crosslinking in bone, but not in ligament or cartilage, in Bruck syndrome: indications for a bone-specific telopeptide lysyl hydroxylase on chromosome 17.

Enhancing versus suppressive effects of stress hormones on skin immune function.

Gene therapy to promote thromboresistance: local overexpression of tissue plasminogen activator to prevent arterial thrombosis in an in vivo rabbit model.

Identification of a Vibrio cholerae RTX toxin gene cluster that is tightly linked to the cholera toxin prophage

Variation in expression of the Haemophilus influenzae HMW adhesins: a prokaryotic system reminiscent of eukaryotes.

The role of presynaptic activity in monocular deprivation: comparison of homosynaptic and heterosynaptic mechanisms.

Molecular and functional remodeling of electrogenic membrane of hypothalamic neurons in response to changes in their input.

A presynaptic role for the ADP ribosylation factor (ARF)-specific GDP/GTP exchange factor msec7-1.

Neuroligin 1 is a postsynaptic cell-adhesion molecule of excitatory synapses.

Spatiotemporal activity patterns of rat cortical neurons predict responses in a conditioned task.

Neurotrophins regulate agrin-induced postsynaptic differentiation.

Glutamate and gamma-aminobutyric acid mediate a heterosynaptic depression at mossy fiber synapses in the hippocampus.

Isometric contraction induces the Ca2+-independent activation of the endothelial nitric oxide synthase.

Role of mitochondrial lactate dehydrogenase and lactate oxidation in the intracellular lactate shuttle.

Unusual carotenoid composition and a new type of xanthophyll cycle in plants.

ARG1 (altered response to gravity) encodes a DnaJ-like protein that potentially interacts with the cytoskeleton.

Antisense-mediated depletion of a potato lipoxygenase reduces wound induction of proteinase inhibitors and increases weight gain of insect pests.

The relationship between the volume of antimicrobial consumption in human communities and the frequency of resistance.

Skeletal and dental morphology supports diphyletic origin of baboons and mandrills.

Year: 1999 Volume: 96 Issue: 2

Year: 1999 Volume: 96 Issue: 1

Year: 1998 Volume: 95

Year: 1997 Volume: 94

Year: 1996 Volume: 93

Year: 1995 Volume: 92

Year: 1994 Volume: 91

Year: 1993 Volume: 90

Year: 1992 Volume: 89

Year: 1991 Volume: 88

Year: 1990 Volume: 87

Year: 1989 Volume: 86

Year: 1988 Volume: 85

Year: 1987 Volume: 84

Year: 1986 Volume: 83

Year: 1985 Volume: 82

Year: 1984 Volume: 81

Year: 1983 Volume: 80

Year: 1982 Volume: 79

Year: 1981 Volume: 78

Year: 1980 Volume: 77

Year: 1979 Volume: 76

Year: 1978 Volume: 75

Year: 1977 Volume: 74

Year: 1976 Volume: 73

Year: 1975 Volume: 72

Year: 1974 Volume: 71

Year: 1973 Volume: 70

Year: 1972 Volume: 69

Year: 1971 Volume: 68

Year: 1970 Volume: 67

Year: 1970 Volume: 66

Year: 1970 Volume: 65

Year: 1969 Volume: 64

Year: 1969 Volume: 63

Year: 1969 Volume: 62

Year: 1968 Volume: 61

Year: 1968 Volume: 60

Year: 1968 Volume: 59

Year: 1967 Volume: 58

Year: 1967 Volume: 57

Year: 1966 Volume: 56

Year: 1966 Volume: 55

Induced fit of a peptide loop of methionyl-tRNA formyltransferase triggered by the initiator tRNA substrate.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Dyson, M. R. Rajbhandary, U. L. Mayer, C. Ramesh, V. Gite, S.
Description	Author Affiliation: Ramesh V ( Department of Biology, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.);
Abstract	A 16-aa insertion loop present in eubacterial methionyl-tRNA formyltransferases (MTF) is critical for specific recognition of the initiator tRNA in Escherichia coli. We have studied the interactions between this region of the E. coli enzyme and initiator methionyl-tRNA (Met-tRNA) by using two complementary protection experiments: protection of MTF against proteolytic cleavage by tRNA and protection of tRNA against nucleolytic cleavage by MTF. The insertion loop in MTF is uniquely sensitive to cleavage by trypsin. We show that the substrate initiator Met-tRNA protects MTF against trypsin cleavage, whereas a formylation-defective mutant initiator Met-tRNA, which binds to MTF with approximately the same affinity, does not. Also, mutants of MTF within the insertion loop (which are defective in formylation) are not protected by the initiator Met-tRNA. Thus, a functional enzyme-substrate complex is necessary for protection of MTF against trypsin cleavage. Along with other data, these results strongly suggest that a segment of the insertion loop, which is exposed and unstructured in MTF, undergoes an induced fit in the functional MTF.Met-tRNA complex but not in the nonfunctional one. Footprinting experiments show that MTF specifically protects the acceptor stem and the 3'-end region of the initiator Met-tRNA against cleavage by double and single strand-specific nucleases. This protection also depends on formation of a functional MTF.Met-tRNA complex. Thus, the insertion loop interacts mostly with the acceptor stem of the initiator Met-tRNA, which contains the critical determinants for formylation.
ISSN	00278424
e-ISSN	10916490
Journal	Proceedings of the National Academy of Sciences of the United States of America
Issue Number	3
Volume Number	96
Language	English
Publisher	National Academy of Sciences
Publisher Date	1999-03-01
Publisher Place	United States
Access Restriction	Open
Subject Keyword	Escherichia Coli Enzymology Hydroxymethyl And Formyl Transferases Chemistry Metabolism Nucleic Acid Conformation RNA, Transfer, Met Trypsin Amino Acid Sequence Conserved Sequence Molecular Sequence Data Mutagenesis, Insertional Research Support, U.S. Gov't, P.H.S. Multidisciplinary
Content Type	Text
Resource Type	Article
Subject	Multidisciplinary

Central Library (ISO-9001:2015 Certified)
Indian Institute of Technology Kharagpur
Kharagpur, West Bengal, India | PIN - 721302

See location in the Map
03222 282435
Mail: support@ndl.gov.in

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in