NDLI: Noninvasive imaging of protein-protein interactions in living subjects by using reporter protein complementation and reconstitution strategies.

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Noninvasive imaging of protein-protein interactions in living subjects by using reporter protein complementation and reconstitution strategies.

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Noninvasive imaging of protein-protein interactions in living subjects by using reporter protein complementation and reconstitution strategies.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Paulmurugan, R. Umezawa, Y. Gambhir, S. S.
Description	Author Affiliation: Paulmurugan R ( Crump Institute for Molecular Imaging, Department of Molecular and Medical Pharmacology, University of California Los Angeles-Jonsson Comprehensive Cancer Center, 90095-1770, USA.);
Abstract	In this study we have developed bioluminescence-imaging strategies to noninvasively and quantitatively image protein-protein interactions in living mice by using a cooled charge-coupled device camera and split reporter technology. We validate both complementation and intein-mediated reconstitution of split firefly luciferase proteins driven by the interaction of two strongly interacting proteins, MyoD and Id. We use transient transfection of cells and image MyoD-Id interaction after induction of gene expression in cell culture and in cells implanted into living mice. Techniques to study protein-protein interactions in living subjects will allow the study of cellular networks, including signal transduction pathways, as well as development and optimization of pharmaceuticals for modulating protein-protein interactions.
ISSN	00278424
e-ISSN	10916490
Journal	Proceedings of the National Academy of Sciences of the United States of America
Issue Number	24
Volume Number	99
Language	English
Publisher	National Academy of Sciences
Publisher Date	2002-11-01
Publisher Place	United States
Access Restriction	Open
Subject Keyword	Genes, Reporter Luciferases MyoD Protein Metabolism Protein Interaction Mapping Recombinant Fusion Proteins Repressor Proteins Transcription Factors Animals COS Cells Cell Line Transplantation Cercopithecus Aethiops Cytomegalovirus Genes, Synthetic Genetic Complementation Test Inhibitor Of Differentiation Protein 1 Kidney Luminescent Measurements Mice Chemistry NF-kappa B Peptide Fragments Genetics Promoter Regions, Genetic Physiology Reproducibility Of Results Transfection Tumor Cells, Cultured Tumor Necrosis Factor-alpha Pharmacology Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S. Multidisciplinary
Content Type	Text
Resource Type	Article
Subject	Multidisciplinary

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