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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Sisson, Lisa Carey, James F. Schultz, Sharon J. Champoux, James J. Fazzio, Thomas G. |
| Description | Author Affiliation: Carey JF ( Department of Microbiology, School of Medicine, University of Washington, Seattle, WA 98195, USA.); |
| Abstract | In cocrystal structures of human topoisomerase I and DNA, the enzyme is tightly clamped around the DNA helix. After cleavage and covalent attachment of the enzyme to the 3' end at the nick, DNA relaxation requires rotation of the DNA helix downstream of the cleavage site. Models based on the cocrystal structure reveal that there is insufficient space in the protein for such DNA rotation without some deformation of the cap and linker regions of the enzyme. Alternatively, it is conceivable that the protein clamp opens to facilitate the rotation process. To distinguish between these two possibilities, we engineered two cysteines into the opposing loops of the 'lips' region of the enzyme, which allowed us to lock the protein via a disulfide crosslink in the closed conformation around the DNA. Importantly, the rate of DNA relaxation when the enzyme was locked on the DNA was comparable to that observed in the absence of the disulfide crosslink. These results indicate that DNA relaxation likely proceeds without extensive opening of the enzyme clamp. |
| ISSN | 00278424 |
| e-ISSN | 10916490 |
| Journal | Proceedings of the National Academy of Sciences of the United States of America |
| Issue Number | 10 |
| Volume Number | 100 |
| Language | English |
| Publisher | National Academy of Sciences |
| Publisher Date | 2003-05-01 |
| Publisher Place | United States |
| Access Restriction | Open |
| Subject Keyword | DNA Topoisomerases, Type I Chemistry Metabolism DNA Amino Acid Substitution Centrifugation, Density Gradient Genetics Isolation & Purification Kinetics Models, Molecular Mutagenesis, Site-Directed Nucleic Acid Conformation Plasmids Protein Conformation Recombinant Proteins Research Support, U.S. Gov't, P.H.S. Multidisciplinary |
| Content Type | Text |
| Resource Type | Article |
| Subject | Multidisciplinary |
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