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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Séraphin, Bertrand Van Dijk, Erwin Le Hir, Hervé |
| Description | Author Affiliation: van Dijk E ( Equipe Labellisée La Ligue, Centre de Génétique Moléculaire, Unité Propre de Recherche 2167, Centre National de la Recherche Scientifique Associée à l'Université Paris VI, Avenue de la Terrasse, 91198 Gif sur Yvette, France.); |
| Abstract | Eukaryotic mRNA degradation proceeds through two main pathways, both involving mRNA cap breakdown. In the 3'-5' mRNA decay pathway, mRNA body degradation generates free m7GpppN that is hydrolyzed by DcpS generating m7GMP. In the 5'-3' pathway, the recently identified human Dcp2 decapping enzyme cleaves the cap of deadenylated mRNAs to produce m7GDP and 5'-phosphorylated mRNA. We investigated mRNA decay in human cell extracts by using a new assay for decapping. We observed that 5'-phosphorylated intermediates resulting from decapping appear after incubation of a substrate RNA in human cell extracts, indicating the presence of an active 5'-3' mRNA decay pathway. Surprisingly, however, the cognate m7GDP product was not detected, whereas abundant amounts of m7GMP were generated. Additional experiments revealed that m7GDP is, unexpectedly, efficiently converted to m7GMP in extracts from various organisms. The factor necessary and sufficient for this reaction was identified as DcpS in both yeast and human. m7GMP is thus a general, pathway-independent, by-product of eukaryotic mRNA decay. m7GDP breakdown should prevent misincorporation of methylated nucleotides in nucleic acids and could generate a unique indicator allowing the cell to monitor mRNA decay. |
| ISSN | 00278424 |
| e-ISSN | 10916490 |
| Journal | Proceedings of the National Academy of Sciences of the United States of America |
| Issue Number | 21 |
| Volume Number | 100 |
| Language | English |
| Publisher | National Academy of Sciences |
| Publisher Date | 2003-10-01 |
| Publisher Place | United States |
| Access Restriction | Open |
| Subject Keyword | Endoribonucleases Metabolism Guanosine Diphosphate Analogs & Derivatives RNA Caps RNA, Messenger Animals Cell Line In Vitro Techniques Models, Biological Chemistry Genetics RNA Stability Recombinant Proteins Saccharomyces Cerevisiae Xenopus Research Support, Non-U.S. Gov't Multidisciplinary |
| Content Type | Text |
| Resource Type | Article |
| Subject | Multidisciplinary |
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