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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Prochiantz, Alain Trembleau, Alain Brunet, Isabelle Nédélec, Stéphane Foucher, Isabelle Bouillot, Colette |
| Description | Author Affiliation: Nédélec S ( Centre National de la Recherche Scientifique, Unité Mixte de Recherche 8542, Neuropharmacology and Development, Ecole Normale Supérieure, 46 Rue d'Ulm, 75005 Paris, France.); |
| Abstract | We report that Emx2 homeogene is expressed at the mRNA and protein levels in the adult mouse olfactory neuroepithelium. As expected for a transcription factor, Emx2 is present in the nucleus of immature and mature olfactory sensory neurons. However, the protein is also detected in the axonal compartment of these neurons, both in the olfactory mucosa axon bundles and in axon terminals within the olfactory bulb. Emx2 axonal staining is heterogeneous, suggesting an association with particles. Subcellular fractionations of olfactory bulb synaptosomes, combined with chemical lesions of olfactory neurons, confirm the presence of Emx2 in axon terminals. Significant amounts of Emx2 protein cosediment with high density synaptosomal subfractions containing eukaryotic translation initiation factor 4E (eIF4E). Nonionic detergents and RNase treatments failed to detach eIF4E and Emx2 from these high-density fractions enriched in vesicles and granular structures. In addition, Emx2 and eIF4E can be coimmunoprecipitated from olfactory mucosa and bulb extracts and interact directly, as demonstrated in pull-down experiments. Emx2 axonal localization, association with high-density particles and interaction with eIF4E strongly suggest that this transcription factor has new nonnuclear functions most probably related to the local control of protein translation in the olfactory sensory neuron axons. Finally, we show that two other brain-expressed homeoproteins, Otx2 and Engrailed 2, also bind eIF4E, indicating that several homeoproteins may modulate eIF4E functions in the developing and adult nervous system. |
| ISSN | 00278424 |
| e-ISSN | 10916490 |
| Journal | Proceedings of the National Academy of Sciences of the United States of America |
| Issue Number | 29 |
| Volume Number | 101 |
| Language | English |
| Publisher | National Academy of Sciences |
| Publisher Date | 2004-07-01 |
| Publisher Place | United States |
| Access Restriction | Open |
| Subject Keyword | Axons Metabolism Eukaryotic Initiation Factor-4E Homeodomain Proteins Olfactory Receptor Neurons Transcription Factors Animals COS Cells Genetics Mice Nerve Tissue Proteins Cytology Otx Transcription Factors Protein Binding Protein Transport RNA, Messenger Recombinant Fusion Proteins Subcellular Fractions Chemistry Synaptosomes Trans-Activators Research Support, Non-U.S. Gov't Multidisciplinary |
| Content Type | Text |
| Resource Type | Article |
| Subject | Multidisciplinary |
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