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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Bash, R. Hinterdorfer, P. Lohr, D. Lindsay, S. M. Gruber, H. Wang, H. Ashcroft, B. Nelson, J. Stroh, C. |
| Description | Author Affiliation: Stroh C ( Department of Physics and Astronomy, Arizona State University, Tempe, AZ 85287, USA.); |
| Abstract | Atomic force microscopy is a powerful and widely used imaging technique that can visualize single molecules and follow processes at the single-molecule level both in air and in solution. For maximum usefulness in biological applications, atomic force microscopy needs to be able to identify specific types of molecules in an image, much as fluorescent tags do for optical microscopy. The results presented here demonstrate that the highly specific antibody-antigen interaction can be used to generate single-molecule maps of specific types of molecules in a compositionally complex sample while simultaneously carrying out high-resolution topographic imaging. Because it can identify specific components, the technique can be used to map composition over an image and to detect compositional changes occurring during a process. |
| ISSN | 00278424 |
| e-ISSN | 10916490 |
| Journal | Proceedings of the National Academy of Sciences of the United States of America |
| Issue Number | 34 |
| Volume Number | 101 |
| Language | English |
| Publisher | National Academy of Sciences |
| Publisher Date | 2004-08-01 |
| Publisher Place | United States |
| Access Restriction | Open |
| Subject Keyword | Antibodies Metabolism Antigens Microscopy, Atomic Force Animals Antigen-Antibody Reactions HeLa Cells Histones Ultrastructure Mammary Tumor Virus, Mouse Genetics Mice Instrumentation Nucleosomes Sensitivity And Specificity Research Support, U.S. Gov't, P.H.S. Multidisciplinary |
| Content Type | Text |
| Resource Type | Article |
| Subject | Multidisciplinary |
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