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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Gibson, Bruce Arnaudeau, Serge Larson, Laura Demaurex, Nicolas Southwick, Frederick Li, Wei Bamburg, James R. Lew, Daniel P. Krause, Ryoko Hao, Binghua |
| Description | Author Affiliation: Larson L ( Department of Medicine, Division of Infectious Diseases, University of Florida College of Medicine, Gainesville, FL 32610, USA.); |
| Abstract | The role of intracellular Ca2+ in the regulation of actin filament assembly and disassembly has not been clearly defined. We show that reduction of intracellular free Ca2+ concentration ([Ca2+]i) to <40 nM in Listeria monocytogenes-infected, EGFP-actin-transfected Madin-Darby canine kidney cells results in a 3-fold lengthening of actin filament tails. This increase in tail length is the consequence of marked slowing of the actin filament disassembly rate, without a significant change in assembly rate. The Ca2+-sensitive actin-severing protein gelsolin concentrates in the Listeria rocket tails at normal resting [Ca2+]i and disassociates from the tails when [Ca2+]i is lowered. Reduction in [Ca2+]i also blocks the severing activity of gelsolin, but not actin-depolymerizing factor (ADF)/cofilin microinjected into Listeria-infected cells. In Xenopus extracts, Listeria tail lengths are also calcium-sensitive, markedly shortening on addition of calcium. Immunodepletion of gelsolin, but not Xenopus ADF/cofilin, eliminates calcium-sensitive actin-filament shortening. Listeria tail length is also calcium-insensitive in gelsolin-null mouse embryo fibroblasts. We conclude that gelsolin is the primary Ca2+-sensitive actin filament recycling protein in the cell and is capable of enhancing Listeria actin tail disassembly at normal resting [Ca2+]i (145 nM). These experiments illustrate the unique and complementary functions of gelsolin and ADF/cofilin in the recycling of actin filaments. |
| ISSN | 00278424 |
| e-ISSN | 10916490 |
| Journal | Proceedings of the National Academy of Sciences of the United States of America |
| Issue Number | 6 |
| Volume Number | 102 |
| Language | English |
| Publisher | National Academy of Sciences |
| Publisher Date | 2005-02-01 |
| Publisher Place | United States |
| Access Restriction | Open |
| Subject Keyword | Actins Metabolism Calcium Egtazic Acid Analogs & Derivatives Gelsolin Listeria Monocytogenes Actin Depolymerizing Factors Genetics Animals Cells, Cultured Chelating Agents Focal Adhesion Kinase 1 Focal Adhesion Protein-Tyrosine Kinases Cytology Mice Mice, Knockout Microfilament Proteins Protein-Tyrosine Kinases Recombinant Fusion Proteins Xenopus Laevis Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S. Multidisciplinary |
| Content Type | Text |
| Resource Type | Article |
| Subject | Multidisciplinary |
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