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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Walisko, Oliver Izsvák, Zsuzsanna Ivics, Zoltán Herold, Steffi Szabó, Kornélia Kaufman, Christopher D. |
| Description | Author Affiliation: Walisko O ( Max Delbrück Center for Molecular Medicine, D-13092 Berlin, Germany.); |
| Abstract | We used the Sleeping Beauty (SB) transposable element as a tool to probe transposon-host cell interactions in vertebrates. The Miz-1 transcription factor was identified as an interactor of the SB transposase in a yeast two-hybrid screen. Through its association with Miz-1, the SB transposase down-regulates cyclin D1 expression in human cells, as evidenced by differential gene expression analysis using microarray hybridization. Down-regulation of cyclin D1 results in a prolonged G(1) phase of the cell cycle and retarded growth of transposase-expressing cells. G(1) slowdown is associated with a decrease of cyclin D1/cdk4-specific phosphorylation of the retinoblastoma protein. Both cyclin D1 down-regulation and the G(1) slowdown induced by the transposase require Miz-1. A temporary G(1) arrest enhances transposition, suggesting that SB transposition is favored in the G(1) phase of the cell cycle, where the nonhomologous end-joining pathway of DNA repair is preferentially active. Because nonhomologous end-joining is required for efficient SB transposition, the transposase-induced G(1) slowdown is probably a selfish act on the transposon's part to maximize the chance for a successful transposition event. |
| ISSN | 00278424 |
| e-ISSN | 10916490 |
| Journal | Proceedings of the National Academy of Sciences of the United States of America |
| Issue Number | 11 |
| Volume Number | 103 |
| Language | English |
| Publisher | National Academy of Sciences |
| Publisher Date | 2006-03-01 |
| Publisher Place | United States |
| Access Restriction | Open |
| Subject Keyword | Cell Cycle Physiology DNA-Binding Proteins Metabolism Transcription Factors Transposases Animals CHO Cells Cricetinae Cyclin D1 Chemistry Genetics Down-Regulation G1 Phase HeLa Cells In Vitro Techniques Kruppel-Like Transcription Factors Oligonucleotide Array Sequence Analysis Phosphorylation Recombinant Fusion Proteins Retinoblastoma Protein Two-Hybrid System Techniques Zinc Fingers Research Support, Non-U.S. Gov't Multidisciplinary |
| Content Type | Text |
| Resource Type | Article |
| Subject | Multidisciplinary |
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