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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Pecorari, Frédéric Schaeffer, Francis Mouratou, Barbara Alzari, Pedro M. Guilvout, Ingrid Tello-manigne, Diana Pugsley, Anthony P. |
| Description | Author Affiliation: Mouratou B ( Unité de Biochimie Structurale, Centre National de la Recherche Scientifique URA2185 and Unité de Génétique Moléculaire, Centre National de la Recherche Scientifique URA2172, Institut Pasteur, 75724 Paris Cedex 15, France.); |
| Abstract | We engineered a class of proteins that binds selected polypeptides with high specificity and affinity. Use of the protein scaffold of Sac7d, belonging to a protein family that binds various ligands, overcomes limitations inherent in the use of antibodies as intracellular inhibitors: it lacks disulfide bridges, is small and stable, and can be produced in large amounts. An in vitro combinatorial/selection approach generated specific, high-affinity (up to 140 pM) binders against bacterial outer membrane secretin PulD. When exported to the Escherichia coli periplasm, they inhibited PulD oligomerization, thereby blocking the type II secretion pathway of which PulD is part. Thus, high-affinity inhibitors of protein function can be derived from Sac7d and can be exported to, and function in, a cell compartment other than that in which they are produced. |
| ISSN | 00278424 |
| e-ISSN | 10916490 |
| Journal | Proceedings of the National Academy of Sciences of the United States of America |
| Issue Number | 46 |
| Volume Number | 104 |
| Language | English |
| Publisher | National Academy of Sciences |
| Publisher Date | 2007-11-01 |
| Publisher Place | United States |
| Access Restriction | Open |
| Subject Keyword | Bacterial Outer Membrane Proteins Antagonists & Inhibitors DNA-Binding Proteins Metabolism Chemistry Enzyme-Linked Immunosorbent Assay Escherichia Coli Models, Molecular Polymerase Chain Reaction Protein Conformation Radioimmunoassay Surface Plasmon Resonance Multidisciplinary |
| Content Type | Text |
| Resource Type | Article |
| Subject | Multidisciplinary |
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