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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Popp, Christian Pauli, Andrea Paro, Renato Beisel, Christian Ringrose, Leonie Strübbe, Gero Schmidt, Alexander |
| Description | Author Affiliation: Strübbe G ( Department of Biosystems Science and Engineering, Swiss Federal Institute of Technology Zürich, Mattenstrasse 26, 4058 Basel, Switzerland.); |
| Abstract | The maintenance of specific gene expression patterns during cellular proliferation is crucial for the identity of every cell type and the development of tissues in multicellular organisms. Such a cellular memory function is conveyed by the complex interplay of the Polycomb and Trithorax groups of proteins (PcG/TrxG). These proteins exert their function at the level of chromatin by establishing and maintaining repressed (PcG) and active (TrxG) chromatin domains. Past studies indicated that a core PcG protein complex is potentially associated with cell type or even cell stage-specific sets of accessory proteins. In order to better understand the dynamic aspects underlying PcG composition and function we have established an inducible version of the biotinylation tagging approach to purify Polycomb and associated factors from Drosophila embryos. This system enabled fast and efficient isolation of Polycomb containing complexes under near physiological conditions, thereby preserving substoichiometric interactions. Novel interacting proteins were identified by highly sensitive mass spectrometric analysis. We found many TrxG related proteins, suggesting a previously unrecognized extent of molecular interaction of the two counteracting chromatin regulatory protein groups. Furthermore, our analysis revealed an association of PcG protein complexes with the cohesin complex and showed that Polycomb-dependent silencing of a transgenic reporter depends on cohesin function. |
| ISSN | 00278424 |
| e-ISSN | 10916490 |
| Journal | Proceedings of the National Academy of Sciences of the United States of America |
| Issue Number | 14 |
| Volume Number | 108 |
| Language | English |
| Publisher | National Academy of Sciences |
| Publisher Date | 2011-04-01 |
| Publisher Place | United States |
| Access Restriction | Open |
| Subject Keyword | Cell Cycle Proteins Metabolism Chromosomal Proteins, Non-Histone Drosophila Proteins Isolation & Purification Drosophila Gene Expression Regulation Physiology Animals Biotinylation Chromatin Chromatography, Gel Genetics Ligands Mass Spectrometry Oligonucleotides Polycomb Repressive Complex 1 Spectrophotometry Research Support, Non-U.S. Gov't Multidisciplinary |
| Content Type | Text |
| Resource Type | Article |
| Subject | Multidisciplinary |
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