NDLI: Measuring steady-state and dynamic endoplasmic reticulum and Golgi $Zn^{2+}$ with genetically encoded sensors

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Measuring steady-state and dynamic endoplasmic reticulum and Golgi $Zn^{2+}$ with genetically encoded sensors

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Measuring steady-state and dynamic endoplasmic reticulum and Golgi $Zn^{2+}$ with genetically encoded sensors

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Park, J. Genevieve Palmer, Amy E. Qin, Yan Dittmer, Philip J. Jansen, Katarina B.
Description	Author Affiliation: Qin Y ( Department of Chemistry and Biochemistry, University of Colorado, Boulder, CO 80309, USA.);
Abstract	Zn(2+) plays essential roles in biology, and cells have adopted exquisite mechanisms for regulating steady-state Zn(2+) levels. Although much is known about total Zn(2+) in cells, very little is known about its subcellular distribution. Yet defining the location of Zn(2+) and how it changes with signaling events is essential for elucidating how cells regulate this essential ion. Here we create fluorescent sensors genetically targeted to the endoplasmic reticulum (ER) and Golgi to monitor steady-state Zn(2+) levels as well as flux of Zn(2+) into and out of these organelles. These studies reveal that ER and Golgi contain a concentration of free Zn(2+) that is 100 times lower than the cytosol. Both organelles take up Zn(2+) when cytosolic levels are elevated, suggesting that the ER and Golgi can sequester elevated cytosolic Zn(2+) and thus have the potential to play a role in influencing Zn(2+) toxicity. ER Zn(2+) homeostasis is perturbed by small molecule antagonists of Ca(2+) homeostasis and ER Zn(2+) is released upon elevation of cytosolic Ca(2+) pointing to potential exchange of these two ions across the ER. This study provides direct evidence that Ca(2+) signaling can influence Zn(2+) homeostasis and vice versa, that Zn(2+) dynamics may modulate Ca(2+) signaling.
ISSN	00278424
e-ISSN	10916490
Journal	Proceedings of the National Academy of Sciences of the United States of America
Issue Number	18
Volume Number	108
Language	English
Publisher	National Academy of Sciences
Publisher Date	2011-05-01
Publisher Place	United States
Access Restriction	Open
Subject Keyword	Calcium Signaling Physiology Endoplasmic Reticulum Metabolism Golgi Apparatus Homeostasis Zinc Amino Acid Sequence Analysis Of Variance Animals Biological Transport Fluorescence Resonance Energy Transfer Galactosyltransferases Genetics HeLa Cells Microscopy, Fluorescence Molecular Sequence Data Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't Multidisciplinary
Content Type	Text
Resource Type	Article
Subject	Multidisciplinary

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