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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Fidalgo, Sara Dell, Anne Berninghausen, Otto Zangrandi, Alice Tonevitsky, Alexander G. Volynski, Kirill E. Rahman, M. Atiqur Hitchen, Paul G. Silva, John-paul Lelianova, Vera G. Ushkaryov, Yuri A. Ermolyuk, Yaroslav S. Vysokov, Nickolai |
| Description | Author Affiliation: Silva JP ( Division of Cell and Molecular Biology, Imperial College London, London SW7 2AZ, United Kingdom.); |
| Abstract | Latrophilin 1 (LPH1), a neuronal receptor of -latrotoxin, is implicated in neurotransmitter release and control of presynaptic Ca(2+). As an 'adhesion G-protein-coupled receptor,' LPH1 can convert cell surface interactions into intracellular signaling. To examine the physiological functions of LPH1, we used LPH1's extracellular domain to purify its endogenous ligand. A single protein of â ¼275 kDa was isolated from rat brain and termed Lasso. Peptide sequencing and molecular cloning have shown that Lasso is a splice variant of teneurin-2, a brain-specific orphan cell surface receptor with a function in neuronal pathfinding and synaptogenesis. We show that LPH1 and Lasso interact strongly and specifically. They are always copurified from rat brain extracts. Coculturing cells expressing LPH1 with cells expressing Lasso leads to their mutual attraction and formation of multiple junctions to which both proteins are recruited. Cells expressing LPH1 form chimerical synapses with hippocampal neurons in cocultures; LPH1 and postsynaptic neuronal protein PSD-95 accumulate on opposite sides of these structures. Immunoblotting and immunoelectron microscopy of purified synapses and immunostaining of cultured hippocampal neurons show that LPH1 and Lasso are enriched in synapses; in both systems, LPH1 is presynaptic, whereas Lasso is postsynaptic. A C-terminal fragment of Lasso interacts with LPH1 and induces Ca(2+) signals in presynaptic boutons of hippocampal neurons and in neuroblastoma cells expressing LPH1. Thus, LPH1 and Lasso can form transsynaptic complexes capable of inducing presynaptic Ca(2+) signals, which might affect synaptic functions. |
| ISSN | 00278424 |
| e-ISSN | 10916490 |
| Journal | Proceedings of the National Academy of Sciences of the United States of America |
| Issue Number | 29 |
| Volume Number | 108 |
| Language | English |
| Publisher | National Academy of Sciences |
| Publisher Date | 2011-07-01 |
| Publisher Place | United States |
| Access Restriction | Open |
| Subject Keyword | Calcium Signaling Physiology Hippocampus Metabolism Nerve Tissue Proteins Genetics Receptors, Cell Surface Receptors, Peptide Synapses Synaptic Transmission Animals Cloning, Molecular Immunoblotting Microscopy, Immunoelectron Molecular Sequence Data Sequence Analysis, DNA Research Support, Non-U.S. Gov't Multidisciplinary |
| Content Type | Text |
| Resource Type | Article |
| Subject | Multidisciplinary |
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