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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Bègue, Aurélien Ogden, David Emiliani, Valentina Anselmi, Francesca Ventalon, Cathie |
| Description | Author Affiliation: Anselmi F ( Wavefront-Engineering Microscopy Group, Neurophysiology and New Microscopies Laboratory, Centre National de la Recherche Scientifique UMR8154, Institut National de la Santé et de la Recherche Médicale U603, Université Paris Descartes, 45 Rue des Saints Pères, F-75006 Paris, France.); |
| Abstract | Access to three-dimensional structures in the brain is fundamental to probe signal processing at multiple levels, from integration of synaptic inputs to network activity mapping. Here, we present an optical method for independent three-dimensional photoactivation and imaging by combination of digital holography with remote-focusing. We experimentally demonstrate compensation of spherical aberration for out-of-focus imaging in a range of at least 300 µm, as well as scanless imaging along oblique planes. We apply this method to perform functional imaging along tilted dendrites of hippocampal pyramidal neurons in brain slices, after photostimulation by multiple spots glutamate uncaging. By bringing extended portions of tilted dendrites simultaneously in-focus, we monitor the spatial extent of dendritic calcium signals, showing a shift from a widespread to a spatially confined response upon blockage of voltage-gated Na(+) channels. |
| ISSN | 00278424 |
| e-ISSN | 10916490 |
| Journal | Proceedings of the National Academy of Sciences of the United States of America |
| Issue Number | 49 |
| Volume Number | 108 |
| Language | English |
| Publisher | National Academy of Sciences |
| Publisher Date | 2011-12-01 |
| Publisher Place | United States |
| Access Restriction | Open |
| Subject Keyword | Dendrites Physiology Holography Imaging, Three-Dimensional Algorithms Animals CA1 Region, Hippocampal Cytology Metabolism Calcium Glutamic Acid Hippocampus Anatomy & Histology Instrumentation Ion Channel Gating Drug Effects Mice Mice, Inbred Strains Microscopy, Fluorescence Patch-Clamp Techniques Pyramidal Cells Sodium Channel Blockers Pharmacology Sodium Channels Tetrodotoxin Research Support, Non-U.S. Gov't Multidisciplinary |
| Content Type | Text |
| Resource Type | Article |
| Subject | Multidisciplinary |
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