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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Durand, Sylvain Bontems, François Uzan, Marc Richard, Graziella |
| Description | Author Affiliation: Durand S ( Institut de Biologie Physico-chimique, Unité Propre de Recherche 9073, Centre National de la Recherche Scientifique affiliated with Université Paris Diderot, 75005 Paris, France.); |
| Abstract | The bacteriophage T4-encoded RegB endoribonuclease is produced during the early stage of phage development and targets mostly (but not exclusively) the Shine-Dalgarno sequences of early genes. In this work, we show that the degradation of RegB-cleaved mRNAs depends on a functional T4 polynucleotide kinase/phosphatase (PNK). The 5'-OH produced by RegB cleavage is phosphorylated by the kinase activity of PNK. This modification allows host RNases G and E, with activity that is strongly stimulated by 5'-monophosphate termini, to attack mRNAs from the 5'-end, causing their destabilization. The PNK-dependent pathway of degradation becomes effective 5 min postinfection, consistent with our finding that several minutes are required for PNK to accumulate after infection. Our work emphasizes the importance of the nature of the 5' terminus for mRNA stability and depicts a pathway of mRNA degradation with 5'- to 3'-polarity in cells devoid of 5'-3' exonucleases. It also ascribes a role for T4 PNK during normal phage development. |
| ISSN | 00278424 |
| e-ISSN | 10916490 |
| Journal | Proceedings of the National Academy of Sciences of the United States of America |
| Issue Number | 18 |
| Volume Number | 109 |
| Language | English |
| Publisher | National Academy of Sciences |
| Publisher Date | 2012-05-01 |
| Publisher Place | United States |
| Access Restriction | Open |
| Subject Keyword | Bacteriophage T4 Metabolism Polynucleotide 5'-Hydroxyl-Kinase RNA, Messenger RNA, Viral Genetics Growth & Development Endoribonucleases Antagonists & Inhibitors Escherichia Coli Proteins Genes, Viral Host-Pathogen Interactions Molecular Sequence Data RNA Stability Viral Proteins Research Support, Non-U.S. Gov't Multidisciplinary |
| Content Type | Text |
| Resource Type | Article |
| Subject | Multidisciplinary |
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