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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Silverman, Scott K. Chandrasekar, Jagadeeswaran |
| Description | Author Affiliation: Chandrasekar J ( Department of Chemistry, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA.); |
| Abstract | Catalytic DNA sequences (deoxyribozymes, DNA enzymes, or DNAzymes) have been identified by in vitro selection for various catalytic activities. Expanding the limits of DNA catalysis is an important fundamental objective and may facilitate practical utility of catalysts that can be obtained from entirely unbiased (random) sequence populations. In this study, we show that DNA can catalyze Zn(2+)-dependent phosphomonoester hydrolysis of tyrosine and serine side chains (i.e., exhibit phosphatase activity). The best deoxyribozyme decreases the half-life for phosphoserine hydrolysis from as high as >10(10) y to <1 h. The phosphatase activity also occurs with nonpeptidic substrates but with reduced efficiency, indicating a preference for phosphopeptides. The newly identified deoxyribozymes can function with multiple turnover using free peptide substrates, have activity in the presence of human cell lysate or BSA, and catalyze dephosphorylation of a larger protein substrate, suggesting broader application of DNA catalysts as artificial phosphatases. |
| ISSN | 00278424 |
| e-ISSN | 10916490 |
| Journal | Proceedings of the National Academy of Sciences of the United States of America |
| Issue Number | 14 |
| Volume Number | 110 |
| Language | English |
| Publisher | National Academy of Sciences |
| Publisher Date | 2013-04-01 |
| Publisher Place | United States |
| Access Restriction | Open |
| Subject Keyword | DNA, Catalytic Metabolism Phosphoric Monoester Hydrolases Catalysis Chromatography, High Pressure Liquid Electrophoresis, Polyacrylamide Gel Half-Life Hydrolysis In Vitro Techniques Mass Spectrometry Oligonucleotides Genetics Phosphorylation Phosphoserine Phosphotyrosine Rosaniline Dyes Research Support, N.I.H., Extramural Research Support, U.S. Gov't, Non-P.H.S. Multidisciplinary |
| Content Type | Text |
| Resource Type | Article |
| Subject | Multidisciplinary |
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