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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Garcia, Hernan G. Bothma, Jacques P. Levine, Michael Schlissel, Gavin Esposito, Emilia Gregor, Thomas |
| Description | Author Affiliation: Bothma JP ( Department of Molecular and Cell Biology, University of California, Berkeley, CA 94720); Garcia HG ( Joseph Henry Laboratories of Physics and.); Esposito E ( Department of Molecular and Cell Biology, University of California, Berkeley, CA 94720); Schlissel G ( Department of Molecular and Cell Biology, University of California, Berkeley, CA 94720); Gregor T ( Joseph Henry Laboratories of Physics andLewis-Sigler Institute for Integrative Genomics, Princeton University, Princeton, NJ 08544 mlevine@berkeley.edu tg2@princeton.edu.); Levine M ( Department of Molecular and Cell Biology, University of California, Berkeley, CA 94720); |
| Abstract | We present the use of recently developed live imaging methods to examine the dynamic regulation of even-skipped (eve) stripe 2 expression in the precellular Drosophila embryo. Nascent transcripts were visualized via MS2 RNA stem loops. The eve stripe 2 transgene exhibits a highly dynamic pattern of de novo transcription, beginning with a broad domain of expression during nuclear cycle 12 (nc12), and progressive refinement during nc13 and nc14. The mature stripe 2 pattern is surprisingly transient, constituting just â ¼15 min of the â ¼90-min period of expression. Nonetheless, this dynamic transcription profile faithfully predicts the limits of the mature stripe visualized by conventional in situ detection methods. Analysis of individual transcription foci reveals intermittent bursts of de novo transcription, with duration cycles of 4-10 min. We discuss a multistate model of transcription regulation and speculate on its role in the dynamic repression of the eve stripe 2 expression pattern during development. |
| ISSN | 00278424 |
| e-ISSN | 10916490 |
| Journal | Proceedings of the National Academy of Sciences of the United States of America |
| Issue Number | 29 |
| Volume Number | 111 |
| Language | English |
| Publisher | National Academy of Sciences |
| Publisher Date | 2014-07-01 |
| Publisher Place | United States |
| Access Restriction | Open |
| Subject Keyword | Drosophila Proteins Genetics Drosophila Melanogaster Embryology Embryo, Nonmammalian Metabolism Gene Expression Regulation, Developmental Homeodomain Proteins Transcription Factors Transcription, Genetic Animals Cell Nucleus RNA, Messenger Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't Multidisciplinary |
| Content Type | Text |
| Resource Type | Article |
| Subject | Multidisciplinary |
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