| Content Provider |
World Health Organization (WHO)-Global Index Medicus |
| Author |
Qin, Yujia Turkarslan, Serdar Zane, Grant M. Wu, Liyou Stahl, David A. Hillesland, Kristina L. Lim, Sujung Wall, Judy D. Elliott, Nicholas Zhou, Jizhong Baliga, Nitin S. Flowers, Jason J. Pinel, Nicolas |
| Description |
Author Affiliation: Hillesland KL ( Biological Sciences, University of Washington Bothell, Bothell, WA 98011); Lim S ( Biological Sciences, University of Washington Bothell, Bothell, WA 98011); Flowers JJ ( Civil and Environmental Engineering, and.); Turkarslan S ( Institute for Systems Biology, Seattle, WA 98109-5234); Pinel N ( Civil and Environmental Engineering, and Institute for Systems Biology, Seattle, WA 98109-5234); Zane GM ( Department of Biochemistry, University of Missouri, Columbia, MO 65211); Elliott N ( Civil and Environmental Engineering, and.); Qin Y ( Institute for Environmental Genomics and Department of Microbiology and Plant Biology, University of Oklahoma, Norman, OK 73019); Wu L ( Institute for Environmental Genomics and Department of Microbiology and Plant Biology, University of Oklahoma, Norman, OK 73019); Baliga NS ( Institute for Systems Biology, Seattle, WA 98109-5234); Zhou J ( Institute for Environmental Genomics and Department of Microbiology and Plant Biology, University of Oklahoma, Norman, OK 73019); Wall JD ( Department of Biochemistry, University of Missouri, Columbia, MO 65211); Stahl DA ( Civil and Environmental Engineering, and.); |
| Abstract |
Many species have evolved to function as specialized mutualists, often to the detriment of their ability to survive independently. However, there are few, if any, well-controlled observations of the evolutionary processes underlying the genesis of new mutualisms. Here, we show that within the first 1,000 generations of initiating independent syntrophic interactions between a sulfate reducer (Desulfovibrio vulgaris) and a hydrogenotrophic methanogen (Methanococcus maripaludis), D. vulgaris frequently lost the capacity to grow by sulfate respiration, thus losing the primary physiological attribute of the genus. The loss of sulfate respiration was a consequence of mutations in one or more of three key genes in the pathway for sulfate respiration, required for sulfate activation (sat) and sulfate reduction to sulfite (apsA or apsB). Because loss-of-function mutations arose rapidly and independently in replicated experiments, and because these mutations were correlated with enhanced growth rate and productivity, gene loss could be attributed to natural selection, even though these mutations should significantly restrict the independence of the evolved D. vulgaris. Together, these data present an empirical demonstration that specialization for a mutualistic interaction can evolve by natural selection shortly after its origin. They also demonstrate that a sulfate-reducing bacterium can readily evolve to become a specialized syntroph, a situation that may have often occurred in nature. |
| ISSN |
00278424 |
| e-ISSN |
10916490 |
| Journal |
Proceedings of the National Academy of Sciences of the United States of America |
| Issue Number |
41 |
| Volume Number |
111 |
| Language |
English |
| Publisher |
National Academy of Sciences |
| Publisher Date |
2014-10-01 |
| Publisher Place |
United States |
| Access Restriction |
Open |
| Subject Keyword |
Desulfovibrio Vulgaris Genetics Directed Molecular Evolution Methanococcus Coculture Techniques Mutation Oxidation-Reduction Phenotype Sulfates Metabolism Symbiosis Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Multidisciplinary |
| Content Type |
Text |
| Resource Type |
Article |
| Subject |
Multidisciplinary |
