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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Plitzko, Jürgen M. Baumeister, Wolfgang Khoshouei, Maryam Danev, Radostin Buijsse, Bart |
| Description | Author Affiliation: Danev R ( Department of Molecular Structural Biology, Max Planck Institute of Biochemistry, 82152 Martinsried, Germany); Buijsse B ( FEI, 5651 GG Eindhoven, The Netherlands); Khoshouei M ( Department of Molecular Structural Biology, Max Planck Institute of Biochemistry, 82152 Martinsried, Germany); Plitzko JM ( Department of Molecular Structural Biology, Max Planck Institute of Biochemistry, 82152 Martinsried, Germany); Baumeister W ( Department of Molecular Structural Biology, Max Planck Institute of Biochemistry, 82152 Martinsried, Germany); |
| Abstract | We describe a phase plate for transmission electron microscopy taking advantage of a hitherto-unknown phenomenon, namely a beam-induced Volta potential on the surface of a continuous thin film. The Volta potential is negative, indicating that it is not caused by beam-induced electrostatic charging. The film must be heated to â ¼ 200 °C to prevent contamination and enable the Volta potential effect. The phase shift is created 'on the fly' by the central diffraction beam eliminating the need for precise phase plate alignment. Images acquired with the Volta phase plate (VPP) show higher contrast and unlike Zernike phase plate images no fringing artifacts. Following installation into the microscope, the VPP has an initial settling time of about a week after which the phase shift behavior becomes stable. The VPP has a long service life and has been used for more than 6 mo without noticeable degradation in performance. The mechanism underlying the VPP is the same as the one responsible for the degradation over time of the performance of thin-film Zernike phase plates, but in the VPP it is used in a constructive way. The exact physics and/or chemistry behind the process causing the Volta potential are not fully understood, but experimental evidence suggests that radiation-induced surface modification combined with a chemical equilibrium between the surface and residual gases in the vacuum play an important role. |
| ISSN | 00278424 |
| e-ISSN | 10916490 |
| Journal | Proceedings of the National Academy of Sciences of the United States of America |
| Issue Number | 44 |
| Volume Number | 111 |
| Language | English |
| Publisher | National Academy of Sciences |
| Publisher Date | 2014-11-01 |
| Publisher Place | United States |
| Access Restriction | Open |
| Subject Keyword | Multidisciplinary |
| Content Type | Text |
| Resource Type | Article |
| Subject | Multidisciplinary |
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