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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Peng, Yi Woodson, Sarah A. Fang, Xianyang Curtis, Joseph E. |
| Description | Author Affiliation: Peng Y ( Cell, Molecular and Developmental Biology and Biophysics Program, Johns Hopkins University, Baltimore, MD 21218); Curtis JE ( Center for Neutron Research, National Institute of Standards and Technology, Gaithersburg, MD 20899); Fang X ( Structural Biophysics Laboratory, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Frederick, MD 21702); Woodson SA ( T. C. Jenkins Department of Biophysics, Johns Hopkins University, Baltimore, MD 21218 swoodson@jhu.edu.); |
| Abstract | The Sm-like protein Hfq (host factor Q-beta phage) facilitates regulation by bacterial small noncoding RNAs (sRNAs) in response to stress and other environmental signals. Here, we present a low-resolution model of Escherichia coli Hfq bound to the rpoS mRNA, a bacterial stress response gene that is targeted by three different sRNAs. Selective 2'-hydroxyl acylation and primer extension, small-angle X-ray scattering, and Monte Carlo molecular dynamics simulations show that the distal face and lateral rim of Hfq interact with three sites in the rpoS leader, folding the RNA into a compact tertiary structure. These interactions are needed for sRNA regulation of rpoS translation and position the sRNA target adjacent to an sRNA binding region on the proximal face of Hfq. Our results show how Hfq specifically distorts the structure of the rpoS mRNA to enable sRNA base pairing and translational control. |
| ISSN | 00278424 |
| e-ISSN | 10916490 |
| Journal | Proceedings of the National Academy of Sciences of the United States of America |
| Issue Number | 48 |
| Volume Number | 111 |
| Language | English |
| Publisher | National Academy of Sciences |
| Publisher Date | 2014-12-01 |
| Publisher Place | United States |
| Access Restriction | Open |
| Subject Keyword | Escherichia Coli Proteins Chemistry Host Factor 1 Protein RNA, Bacterial RNA, Messenger Genetics Metabolism Molecular Dynamics Simulation Monte Carlo Method Nucleic Acid Conformation Protein Binding Protein Structure, Tertiary RNA, Small Untranslated Scattering, Small Angle Sigma Factor X-Ray Diffraction Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Multidisciplinary |
| Content Type | Text |
| Resource Type | Article |
| Subject | Multidisciplinary |
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