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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Wohlschlegel, James A. Ausin, Israel Greenberg, Maxim V. C. Groth, Martin Vashisht, Ajay A. Stroud, Hume Jacobsen, Steven E. Feng, Suhua |
| Description | Author Affiliation: Groth M ( Department of Molecular, Cell, and Developmental Biology.); Stroud H ( Department of Molecular, Cell, and Developmental Biology.); Feng S ( Department of Molecular, Cell, and Developmental Biology, Eli & Edythe Broad Center of Regenerative Medicine & Stem Cell Research, Howard Hughes Medical Institute, University of California, Los Angeles, CA 90095); Greenberg MV ( Department of Molecular, Cell, and Developmental Biology.); Vashisht AA ( Department of Biological Chemistry, David Geffen School of Medicine.); Wohlschlegel JA ( Department of Biological Chemistry, David Geffen School of Medicine.); Jacobsen SE ( Department of Molecular, Cell, and Developmental Biology, Eli & Edythe Broad Center of Regenerative Medicine & Stem Cell Research, Howard Hughes Medical Institute, University of California, Los Angeles, CA 90095); Ausin I ( Basic Forestry and Biotechnology Center, Fujian Agriculture and Forestry University, Fujian, Fuzhou 350002, China jacobsen@ucla.edu israel.ausin@gmail.com.); |
| Abstract | DNA methylation in Arabidopsis thaliana is maintained by at least four different enzymes: DNA METHYLTRANSFERASE1 (MET1), CHROMOMETHYLASE3 (CMT3), DOMAINS REARRANGED METHYLTRANSFERASE2 (DRM2), and CHROMOMETHYLASE2 (CMT2). However, DNA methylation is established exclusively by the enzyme DRM2, which acts in the RNA-directed DNA methylation (RdDM) pathway. Some RdDM components belong to gene families and have partially redundant functions, such as the endoribonucleases DICER-LIKE 2, 3, and 4, and INVOLVED IN DE NOVO2 (IDN2) interactors IDN2-LIKE 1 and 2. Traditional mutagenesis screens usually fail to detect genes if they are redundant, as the loss of one gene can be compensated by a related gene. In an effort to circumvent this issue, we used coexpression data to identify closely related genes that are coregulated with genes in the RdDM pathway. Here we report the discovery of two redundant proteins, SNF2-RING-HELICASE–LIKE1 and -2 (FRG1 and -2) that are putative chromatin modifiers belonging to the SNF2 family of helicase-like proteins. Analysis of genome-wide bisulfite sequencing shows that simultaneous mutations of FRG1 and -2 cause defects in methylation at specific RdDM targeted loci. We also show that FRG1 physically associates with Su(var)3-9–related SUVR2, a known RdDM component, in vivo. Combined, our results identify FRG1 and FRG2 as previously unidentified components of the RdDM machinery. |
| ISSN | 00278424 |
| e-ISSN | 10916490 |
| Journal | Proceedings of the National Academy of Sciences of the United States of America |
| Issue Number | 49 |
| Volume Number | 111 |
| Language | English |
| Publisher | National Academy of Sciences |
| Publisher Date | 2014-12-01 |
| Publisher Place | United States |
| Access Restriction | Open |
| Subject Keyword | Arabidopsis Proteins Metabolism Arabidopsis Genetics Chromatin Chemistry Chromosomal Proteins, Non-Histone DNA Methylation RNA, Plant Chromatin Assembly And Disassembly Epigenesis, Genetic Gene Expression Regulation, Plant Gene Silencing Mutagenesis Mutation RNA Interference RNA, Small Interfering Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't Multidisciplinary |
| Content Type | Text |
| Resource Type | Article |
| Subject | Multidisciplinary |
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