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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Kim, Jiyeon Connell, Jodi L. Bard, Allen J. Shear, Jason B. Whiteley, Marvin |
| Description | Author Affiliation: Connell JL ( Department of Molecular Biosciences, Institute of Cellular and Molecular Biology, Center for Infectious Disease and.); Kim J ( Department of Chemistry, The University of Texas at Austin, Austin, TX 78712.); Shear JB ( Department of Chemistry, The University of Texas at Austin, Austin, TX 78712.); Bard AJ ( Department of Chemistry, The University of Texas at Austin, Austin, TX 78712 ajbard@mail.utexas.edu mwhiteley@austin.utexas.edu.); Whiteley M ( Department of Molecular Biosciences, Institute of Cellular and Molecular Biology, Center for Infectious Disease and ajbard@mail.utexas.edu mwhiteley@austin.utexas.edu.); |
| Abstract | Microbes frequently live in nature as small, densely packed aggregates containing â ¼10(1)-10(5) cells. These aggregates not only display distinct phenotypes, including resistance to antibiotics, but also, serve as building blocks for larger biofilm communities. Aggregates within these larger communities display nonrandom spatial organization, and recent evidence indicates that this spatial organization is critical for fitness. Studying single aggregates as well as spatially organized aggregates remains challenging because of the technical difficulties associated with manipulating small populations. Micro-3D printing is a lithographic technique capable of creating aggregates in situ by printing protein-based walls around individual cells or small populations. This 3D-printing strategy can organize bacteria in complex arrangements to investigate how spatial and environmental parameters influence social behaviors. Here, we combined micro-3D printing and scanning electrochemical microscopy (SECM) to probe quorum sensing (QS)-mediated communication in the bacterium Pseudomonas aeruginosa. Our results reveal that QS-dependent behaviors are observed within aggregates as small as 500 cells; however, aggregates larger than 2,000 bacteria are required to stimulate QS in neighboring aggregates positioned 8 µm away. These studies provide a powerful system to analyze the impact of spatial organization and aggregate size on microbial behaviors. |
| ISSN | 00278424 |
| e-ISSN | 10916490 |
| Journal | Proceedings of the National Academy of Sciences of the United States of America |
| Issue Number | 51 |
| Volume Number | 111 |
| Language | English |
| Publisher | National Academy of Sciences |
| Publisher Date | 2014-12-01 |
| Publisher Place | United States |
| Access Restriction | Open |
| Subject Keyword | Bacterial Physiological Phenomena Microscopy, Electrochemical, Scanning Printing, Three-Dimensional Quorum Sensing Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Multidisciplinary |
| Content Type | Text |
| Resource Type | Article |
| Subject | Multidisciplinary |
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