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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Yeola, Avani Wong, Jason W. H. Hesson, Luke B. Palu, Cintia Walkley, Carl R. Kang, Young Chan Grey, Shane T. Nasrallah, Rabab Unnikrishnan, Ashwin Whan, Renee Hardy, Philip Zarzour, Peter Knezevic, Kathy Mobbs, Ralph Macmillan, Alex Delerue, Fabien Beck, Dominik Ittner, Lars Carnell, Michael Qiao, Qiao Boelen, Lies Villanueva, Jeanette E. Purton, Louise E. Nunez, Andrea C. Ward, Robyn L. Oliver, Rema A. Walsh, William Chandrakanthan, Vashe Kwan, Jair C. Yu, Yan Gladbach, Amadeus Pimanda, John E. |
| Description | Author Affiliation: Chandrakanthan V ( Lowy Cancer Research Centre, University of New South Wales (UNSW) Australia, Sydney, NSW 2052, Australia); Yeola A ( Lowy Cancer Research Centre, University of New South Wales (UNSW) Australia, Sydney, NSW 2052, Australia); Kwan JC ( Lowy Cancer Research Centre, University of New South Wales (UNSW) Australia, Sydney, NSW 2052, Australia); Oliver RA ( The Prince of Wales Clinical School, UNSW Australia, Sydney, NSW 2052, Australia); Qiao Q ( Lowy Cancer Research Centre, University of New South Wales (UNSW) Australia, Sydney, NSW 2052, Australia); Kang YC ( Lowy Cancer Research Centre, University of New South Wales (UNSW) Australia, Sydney, NSW 2052, Australia); Zarzour P ( Lowy Cancer Research Centre, University of New South Wales (UNSW) Australia, Sydney, NSW 2052, Australia); Beck D ( Lowy Cancer Research Centre, University of New South Wales (UNSW) Australia, Sydney, NSW 2052, Australia); Boelen L ( Lowy Cancer Research Centre, University of New South Wales (UNSW) Australia, Sydney, NSW 2052, Australia); Unnikrishnan A ( Lowy Cancer Research Centre, University of New South Wales (UNSW) Australia, Sydney, NSW 2052, Australia); Villanueva JE ( Garvan Institute of Medical Research, Darlinghurst, NSW 2010, Australia); Nunez AC ( Lowy Cancer Research Centre, University of New South Wales (UNSW) Australia, Sydney, NSW 2052, Australia); Knezevic K ( Lowy Cancer Research Centre, University of New South Wales (UNSW) Australia, Sydney, NSW 2052, Australia); Palu C ( Lowy Cancer Research Centre, University of New South Wales (UNSW) Australia, Sydney, NSW 2052, Australia); Nasrallah R ( Lowy Cancer Research Centre, University of New South Wales (UNSW) Australia, Sydney, NSW 2052, Australia); Carnell M ( Mark Wainwright Analytical Centre, UNSW Australia, Sydney, NSW 2052, Australia); Macmillan A ( Mark Wainwright Analytical Centre, UNSW Australia, Sydney, NSW 2052, Australia); Whan R ( Mark Wainwright Analytical Centre, UNSW Australia, Sydney, NSW 2052, Australia); Yu Y ( The Prince of Wales Clinical School, UNSW Australia, Sydney, NSW 2052, Australia); Hardy P ( Cyto Labs Pty. Ltd. Australia, School of Medicine and Pharmacology, University of Western Australia, Fremantle, WA 6102, Australia); Grey ST ( Garvan Institute of Medical Research, Darlinghurst, NSW 2010, Australia); Gladbach A ( Dementia Research Unit, Department of Anatomy, School of Medical Sciences, Faculty of Medicine, University of New South Wales, Sydney, NSW 2052, Australia); Delerue F ( Dementia Research Unit, Department of Anatomy, School of Medical Sciences, Faculty of Medicine, University of New South Wales, Sydney, NSW 2052, Australia); Ittner L ( Dementia Research Unit, Department of Anatomy, School of Medical Sciences, Faculty of Medicine, University of New South Wales, Sydney, NSW 2052, Australia); Mobbs R ( Department of Neurosurgery, The Prince of Wales Hospital, Sydney, NSW 2032, Australia); Walkley CR ( St. Vincent's Institute of Medical Research, The University of Melbourne, Fitzroy, VIC 3065, Australia); Purton LE ( St. Vincent's Institute of Medical Research, The University of Melbourne, Fitzroy, VIC 3065, Australia); Ward RL ( Lowy Cancer Research Centre, University of New South Wales (UNSW) Australia, Sydney, NSW 2052, Australia); Wong JW ( Lowy Cancer Research Centre, University of New South Wales (UNSW) Australia, Sydney, NSW 2052, Australia); Hesson LB ( Lowy Cancer Research Centre, University of New South Wales (UNSW) Australia, Sydney, NSW 2052, Australia); Walsh W ( The Prince of Wales Clinical School, UNSW Australia, Sydney, NSW 2052, Australia); Pimanda JE ( Lowy Cancer Research Centre, University of New South Wales (UNSW) Australia, Sydney, NSW 2052, Australia); |
| Abstract | Current approaches in tissue engineering are geared toward generating tissue-specific stem cells. Given the complexity and heterogeneity of tissues, this approach has its limitations. An alternate approach is to induce terminally differentiated cells to dedifferentiate into multipotent proliferative cells with the capacity to regenerate all components of a damaged tissue, a phenomenon used by salamanders to regenerate limbs. 5-Azacytidine (AZA) is a nucleoside analog that is used to treat preleukemic and leukemic blood disorders. AZA is also known to induce cell plasticity. We hypothesized that AZA-induced cell plasticity occurs via a transient multipotent cell state and that concomitant exposure to a receptive growth factor might result in the expansion of a plastic and proliferative population of cells. To this end, we treated lineage-committed cells with AZA and screened a number of different growth factors with known activity in mesenchyme-derived tissues. Here, we report that transient treatment with AZA in combination with platelet-derived growth factor–AB converts primary somatic cells into tissue-regenerative multipotent stem (iMS) cells. iMS cells possess a distinct transcriptome, are immunosuppressive, and demonstrate long-term self-renewal, serial clonogenicity, and multigerm layer differentiation potential. Importantly, unlike mesenchymal stem cells, iMS cells contribute directly to in vivo tissue regeneration in a context-dependent manner and, unlike embryonic or pluripotent stem cells, do not form teratomas. Taken together, this vector-free method of generating iMS cells from primary terminally differentiated cells has significant scope for application in tissue regeneration. |
| ISSN | 00278424 |
| e-ISSN | 10916490 |
| Journal | Proceedings of the National Academy of Sciences of the United States of America |
| Issue Number | 16 |
| Volume Number | 113 |
| Language | English |
| Publisher | National Academy of Sciences |
| Publisher Date | 2016-04-01 |
| Publisher Place | United States |
| Access Restriction | Open |
| Subject Keyword | Azacitidine Pharmacology Cellular Reprogramming Induced Pluripotent Stem Cells Metabolism Mesenchymal Stromal Cells Platelet-Derived Growth Factor Animals Cells, Cultured Cytology Mice Mice, Transgenic Organ Specificity Physiology Research Support, Non-U.S. Gov't Multidisciplinary |
| Content Type | Text |
| Resource Type | Article |
| Subject | Multidisciplinary |
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