NDLI: Self-assembly of electro-active protein architectures on electrodes for the construction of biomimetic signal chains

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Dronov, Roman Lisdat, Fred Scheller, Frieder W. Kurth, Dirk G. Möhwald, Helmuth
Description	Author Affiliation: Lisdat F ( Wildau University of Applied Sciences, Biosystems Technology, Bahnhofstr. 1, 15745, Wildau, Germany. flisdat@igw.tfh-wildau.de);
Abstract	The layer-by-layer adsorption technique based on the consecutive deposition of oppositely charged species is suitable for the preparation of protein multilayers with fully electro-active protein molecules. The methodology was established with cytochrome c and the polyelectrolyte sulfonated polyaniline (PASA). The technique is also useful for the construction of bi- protein architectures confining protein – protein communication to an electrode. Following natural examples of protein complexes with defined signal transfer, cytochrome c was arranged with enzymes such as xanthine oxidase, bilirubin oxidase, laccase, and sulfite oxidase in self-assembled multilayer architectures. Thus, biomimetic signal chains from the enzyme substrate via the enzyme and cytochrome c towards the electrode can be established. Communication between proteins immobilised in multiple layers on the electrode can be achieved by in situ generation of small shuttle molecules or more advantageously by direct interprotein electron transfer . This allows the construction of new sensing electrodes, the properties of which can be tuned by the number of deposited protein layers. The mechanism of electron transfer within such protein assemblies on gold electrodes will be discussed.
ISSN	13597345
Journal	Chemical Communications
Issue Number	3
e-ISSN	1364548X
Language	English
Publisher	Royal Society of Chemistry
Publisher Date	2009-02-01
Publisher Place	Great Britain (UK)
Access Restriction	Subscribed
Subject Keyword	Biomimetics Methods Proteins Chemistry Aniline Compounds Cytochromes c Electrochemistry Electrodes Polymers Sulfonic Acids Journal Article Research Support, Non-U.S. Gov't
Content Type	Text
Resource Type	Article
Subject	Ceramics and Composites Chemistry Surfaces, Coatings and Films Materials Chemistry Metals and Alloys Electronic, Optical and Magnetic Materials Catalysis

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