NDLI: Rational design of boron dipyrromethene (BODIPY)-based photobleaching-resistant fluorophores applicable to a protein dynamics study

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Komatsu, Toru Terai, Takuya Nagano, Tetsuo Hanaoka, Kenjiro Takeda, Aoi Mineno, Tomoko Oushiki, Daihi Ueno, Tasuku Miyamura, Masaki Urano, Yasuteru
Description	Author Affiliation: Komatsu T ( Graduate School of Pharmaceutical Sciences, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo, 113-0033, Japan.);
Abstract	We studied the photobleaching of a library of boron dipyrromethene (BODIPY) derivatives with a range of electron densities, and found that the photobleaching rate is influenced by the electron-withdrawing capacity of the substituents. Electron-deficient BODIPYs generated less singlet oxygen, were less reactive to singlet oxygen, and were highly resistant to photobleaching. We confirmed the utility of one of these fluorophores, $2,6-diCO_{2}R-BDP,$ for visualizing EGF receptor dynamics in cells expressing an SNAP-tagged EGF receptor.
ISSN	13597345
Volume Number	47
Journal	Chemical Communications
Issue Number	36
e-ISSN	1364548X
Language	English
Publisher	Royal Society of Chemistry
Publisher Date	2011-08-01
Publisher Place	Great Britain (UK)
Access Restriction	Subscribed
Subject Keyword	Boron Compounds Chemistry Fluorescent Dyes Receptor, Epidermal Growth Factor Animals COS Cells Cercopithecus Aethiops Microscopy, Confocal Molecular Dynamics Simulation Photobleaching Reactive Oxygen Species Spectrometry, Fluorescence Journal Article
Content Type	Text
Resource Type	Article
Subject	Ceramics and Composites Chemistry Surfaces, Coatings and Films Materials Chemistry Metals and Alloys Electronic, Optical and Magnetic Materials Catalysis

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