NDLI: Distance-determined sensitivity in attenuated total reflection-surface enhanced infrared absorption spectroscopy: aptamer–antigen compared to antibody

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Bao, Wen-jing Wang, Kang Wang, Zhen-lin Zhao, Yun Wang, Min Xia, Xing-hua Li, Jian Yan, Zhen-dong
Description	Author Affiliation: Bao WJ ( State Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing, 210093, China. xhxia@nju.edu.cn.);
Abstract	Distance-dependent signal intensity in immunoassay by attenuated total reflection-surface enhanced infrared absorption spectroscopy is demonstrated by controlling the distance of target proteins away from the enhancement substrate. Based on this optical near-field effect, sensitive detection of protein molecules with a detection limit of 0.6 nM and investigation of the kinetics and thermodynamics of protein–aptamer/antibody interactions can be achieved.
ISSN	13597345
Volume Number	50
Journal	Chemical Communications
Issue Number	58
e-ISSN	1364548X
Language	English
Publisher	Royal Society of Chemistry
Publisher Date	2014-06-01
Publisher Place	Great Britain (UK)
Access Restriction	Subscribed
Subject Keyword	Antibodies Immunology Antigens Aptamers, Peptide Immunoassay Methods Proteins Analysis Spectrophotometry, Infrared L-Selectin Thermodynamics Comparative Study Journal Article Research Support, Non-U.S. Gov't Chemistry
Content Type	Text
Resource Type	Article
Subject	Ceramics and Composites Chemistry Surfaces, Coatings and Films Materials Chemistry Metals and Alloys Electronic, Optical and Magnetic Materials Catalysis

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in