NDLI: Applications of digital holographic microscopy in therapeutic evaluation of Chinese herbal medicines

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Cheng, Chau-jern Chang, Chun-yen Yu, Yu-chen Wu, Chung-hsin Lai, Xin-ji
Abstract	Therapeutic use of Chinese herbal medicines (CHMs) is a new approach to treat neurodegenerative diseases, such as Alzheimer’s disease and Parkinson’s disease. The detection of soma volume and neurite outgrowth of living neurons is a highly relevant biomarker related to various application fields, including therapy efficacy and drug safety evaluation. Through the use of digital holographic microscopy (DHM), we may evaluate the therapeutic effect of CHMs in curing neurodegeneration. Panax ginseng has been used in traditional Chinese herbal medicine for centuries. In this study, DHM is applied to monitor the three-dimensional morphology change of retinoic acid-induced human neuroblastoma SH-SY5Y cells during Panax ginseng treatment. We demonstrate the capability of DHM to detect noninvasively SH-SY5Y cell apoptosis and rescue through the measurement of neuronal volume and neurite outgrowth regulation without any labeling reagent. Through DHM, we observed the phase images of the rapidly shrinking cells with decreasing soma volume and shortening neurite outgrowth during glutamate treatments. Then shrinkage in glutamate-induced cells is significantly alleviated during Panax ginseng treatment. The results through DHM are consistent with the result from MTT assay for assessing cell viability during Panax ginseng treatment. Thus, we suggest that application of DHM for measuring soma volume and neurite outgrowth of living neurons may be one appropriate therapeutic evaluation for CHMs.
ISSN	1559128X
Journal	Applied Optics
Issue Number	27
Volume Number	53
e-ISSN	21553165
Language	English
Publisher	Optical Society of America
Publisher Date	2014-10-01
Publisher Place	United States
Access Restriction	Subscribed
Subject Keyword	Drug Monitoring Methods Drugs, Chinese Herbal Pharmacology Holography Microscopy Neuroblastoma Drug Therapy Pathology Panax Chemistry Cell Line, Tumor Drug Evaluation, Preclinical Humans Imaging, Three-Dimensional Phytotherapy Signal Processing, Computer-Assisted Technology, Pharmaceutical Treatment Outcome Journal Article Research Support, Non-U.S. Gov't Optics
Content Type	Text
Resource Type	Article
Subject	Atomic and Molecular Physics, and Optics Electrical and Electronic Engineering

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