NDLI: Overexpression of PSF1 is correlated with poor prognosis in hepatocellular carcinoma patients.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Zhou, Lei Sun, Xue-jun Liu, Chang Wu, Qi-fei Tai, Ming-hui Wei, Ji-chao Lei, Lei Meng, Fan-di Qu, Kai Xu, Jia
Description	Author Affiliation: Zhou L ( Department of Hepatobiliary Surgery, The First Affiliated Hospital, School of Medicine, Xi'an Jiao tong University, Xi'an - PR China.)
Abstract	BACKGROUND: PSF1 is a subunit of the GINS complex which is essential for establishment of DNA replication forks, and the progression of the replisome. Previous studies have shown a close relationship between PSF1 and cell cycle in the proliferation of immature cells as well as tumors. The purpose of this study was to measure PSF1 expression in hepatocellular carcinoma (HCC) tissues, and determine the effects of down-regulation of PSF1 expression on growth of cancer cells, the cell cycle, apoptosis and cell invasiveness. METHODS: Samples from 137 HCC tissues, 67 from adjacent nontumor tissue and 15 from normal liver were studied using immunochemistry. The HepG2 cell line was used for knockdown experiments studied by RT-PCR, real-time PCR, apoptosis and invasiveness assays. RESULTS: PSF1 was overexpressed in HCC tissues compared with normal liver tissues. High PSF1 expression correlated with a more aggressive phenotype as well as worse prognosis in HCC patients. Knockdown of PSF1 expression using small interfering RNA (siRNA) slowed the growth of cancer cell by suppressing the cell cycle progression as well as increasing apoptosis, especially early apoptosis. In addition, the invasiveness of HepG2 cells was also reduced by down-regulation of PSF1. CONCLUSIONS: These results suggest that the inhibition of PSF1 might provide new therapeutic approaches for HCC.
File Format	HTM / HTML
ISSN	03936155
Issue Number	1
Volume Number	30
e-ISSN	17246008
Journal	The International Journal of Biological Markers
Language	English
Publisher	Sage Publications
Publisher Date	2015-02-24
Publisher Place	Italy
Access Restriction	Subscribed
Subject Keyword	Discipline Biochemistry Discipline Oncology Atp-binding Cassette Transporters Metabolism Tumor Markers, Biological Carcinoma, Hepatocellular Liver Neoplasms Mortality Pathology Cell Proliferation Female Gene Expression Hep G2 Cells Humans Kaplan-meier Estimate Male Middle Aged Prognosis Roc Curve Journal Article Research Support, Non-u.s. Gov't
Content Type	Text
Resource Type	Article
Subject	Clinical Biochemistry Cancer Research Pathology and Forensic Medicine Oncology

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