NDLI: 24-hour rhythms in oxidative stress during hepatocarcinogenesis in rats: effect of melatonin or alpha-ketoglutarate.

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24-hour rhythms in oxidative stress during hepatocarcinogenesis in rats: effect of melatonin or alpha-ketoglutarate.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Subramanian, Perumal Dakshayani, Kadiyala Babu Pandi-Perumal, Seithikurippu R. Trakht, Ilya Cardinali, Daniel P.
Description	Country affiliation: India Author Affiliation: Subramanian P ( Department of Biochemistry and Biotechnology, Faculty of Science, Annamalai University, Annamalainagar, Tamil Nadu, India.)
Abstract	To compare the effects of alpha-ketoglutarate (alpha-KG) and melatonin on 24-h rhythmicity of oxidative stress in N-nitrosodiethylamine (NDEA)-injected Wistar male rats, melatonin (5 mg/kg i.p.) or alpha-KG (2 g/kg through an intragastric tube) was given daily for 20 weeks. In blood collected at 6 time points during a 24-h period, serum activity of aspartate transaminase (AST) and alanine transaminase (ALT) and the levels of alpha-fetoprotein (alpha-FP) were measured as markers of liver function. To assess lipid peroxidation and the antioxidant status, plasma levels of thiobarbituric acid reactive substances (TBARS) and of reduced glutathione (GSH) were measured, together with the activity of erythrocyte superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx) and glutathione S-transferase (GST). NDEA augmented mesor and amplitude of rhythms in AST and ALT activity and plasma alpha-FP levels and mesor values of plasma TBARS, while decreasing mesor values of plasma GSH and erythrocyte SOD, CAT, GPx and GST. Acrophases were delayed by NDEA in all cases except for alpha-FP rhythm, which became phase-advanced. Co-administration of melatonin or alpha-KG partially counteracted the effects of NDEA. Melatonin decreased mesor of plasma TBARS and augmented mesor of SOD activity. The results indicate that melatonin and alpha-KG are effective in protecting from NDEA-induced perturbation of 24-h rhythms in oxidative stress. Melatonin augmented antioxidant defense in rats.
File Format	HTM / HTML
ISSN	13510002
e-ISSN	17432928
Journal	Redox Report
Issue Number	2
Volume Number	13
Language	English
Publisher	Taylor & Francis
Publisher Date	2008-01-01
Publisher Place	Great Britain (UK)
Access Restriction	Subscribed
Subject Keyword	Discipline Metabolism Discipline Biochemistry Antioxidants Pharmacology Carcinoma, Hepatocellular Drug Therapy Circadian Rhythm Drug Effects Ketoglutaric Acids Liver Neoplasms, Experimental Melatonin Oxidative Stress Alanine Transaminase Blood Animals Aspartate Aminotransferases Chemically Induced Pathology Catalase Metabolism Diethylnitrosamine Toxicity Erythrocytes Enzymology Glutathione Glutathione Peroxidase Glutathione Transferase Lipid Peroxidation Rats, Wistar Superoxide Dismutase Thiobarbituric Acid Reactive Substances Alpha-fetoproteins
Content Type	Text
Resource Type	Article
Subject	Biochemistry (medical) Cell Biology Physiology Biochemistry Clinical Biochemistry

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