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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Saravanan, R. Vairamani, S. Shanmugam, A. |
| Description | Country affiliation: India Author Affiliation: Saravanan R ( Centre of Advanced Study in Marine Biology, Annamalai University, Parangipettai, Tamil Nadu, India. saran_prp@yahoo.com) |
| Abstract | The extracted unfractionated glycosaminoglycans (GAGs) obtained from the marine clam Meretrix meretrix were fractionated by ion-exchange (Amberlite IRA-900 and 120) chromatography. The fractionated sample activity was determined through azure-A by metachromatic activity and agarose gel electrophoresis. The active fractionated sample molecular mass was determined through gradient polyacrylamide gel electrophoresis (PAGE). The structural characterization of low-molecular-weight GAGs was analyzed by Fourier transform infrared (FT-IR) and ¹H-nuclear magnetic resonance (NMR) spectroscopy. The Activated partial thromboplastin time (APTT) of fractionated heparin-like glycosminoglycan is 72 IU/mg and it has a molecular mass of 15,000 Da. The disaccharide profiles, such as uronic acid 15.31%, hexosamine 24.61%, and sulfate content 11.7%, were also determined. The results of this study suggest that the GAGs from M. meretrix could be an alternative source of heparin. |
| File Format | HTM / HTML |
| ISSN | 10826068 |
| Issue Number | 4 |
| Volume Number | 40 |
| e-ISSN | 15322297 |
| Journal | Preparative Biochemistry and Biotechnology |
| Language | English |
| Publisher | Taylor & Francis |
| Publisher Date | 2010-01-01 |
| Publisher Place | Great Britain (UK) |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Discipline Biochemistry Discipline Biotechnology Anticoagulants Isolation & Purification Pharmacology Bivalvia Chemistry Blood Coagulation Drug Effects Glycosaminoglycans Animals Electrophoresis, Polyacrylamide Gel Humans Nuclear Magnetic Resonance, Biomolecular Partial Thromboplastin Time Spectroscopy, Fourier Transform Infrared Journal Article |
| Content Type | Text |
| Resource Type | Article |
| Subject | Medicine Biochemistry Biotechnology |
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