NDLI: Improved cellulase production by Botryosphaeria rhodina from OPEFB at low level moisture condition through statistical optimization.

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Improved cellulase production by Botryosphaeria rhodina from OPEFB at low level moisture condition through statistical optimization.

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Improved cellulase production by Botryosphaeria rhodina from OPEFB at low level moisture condition through statistical optimization.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Bahrin, E. K. Ibrahim, M. F. Abd Razak, M. N. Abd-Aziz, S. Shah, U. K. Md Alitheen, N. Salleh, M. Md
Description	Country affiliation: Malaysia Author Affiliation: Bahrin EK ( Bioprocess Technology Department, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, Malaysia.)
Abstract	The response surface method was applied in this study to improve cellulase production from oil palm empty fruit bunch (OPEFB) by Botryosphaeria rhodina. An experimental design based on a two-level factorial was employed to screen the significant environmental factors for cellulase production. The locally isolated fungus Botryosphaeria rhodina was cultivated on OPEFB under solid-state fermentation (SSF). From the analysis of variance (ANOVA), the initial moisture content, amount of substrate, and initial pH of nutrient supplied in the SSF system significantly influenced cellulase production. Then the optimization of the variables was done using the response surface method according to central composite design (CCD). Botryosphaeria rhodina exhibited its best performance with a high predicted value of FPase enzyme production (17.95 U/g) when the initial moisture content was at 24.32%, initial pH of nutrient was 5.96, and 3.98 g of substrate was present. The statistical optimization from actual experiment resulted in a significant increment of FPase production from 3.26 to 17.91 U/g (5.49-fold). High cellulase production at low moisture content is a very rare condition for fungi cultured in solid-state fermentation.
File Format	HTM / HTML
ISSN	10826068
Issue Number	2
Volume Number	42
e-ISSN	15322297
Journal	Preparative Biochemistry and Biotechnology
Language	English
Publisher	Taylor & Francis
Publisher Date	2012-01-01
Publisher Place	Great Britain (UK)
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Discipline Biochemistry Discipline Biotechnology Ascomycota Enzymology Cellulase Metabolism Fermentation Industrial Microbiology Methods Plant Oils Analysis Of Variance Isolation & Purification Models, Statistical Journal Article Research Support, Non-u.s. Gov't
Content Type	Text
Resource Type	Article
Subject	Medicine Biochemistry Biotechnology

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