NDLI: Statistical approach for the enhanced production of cold-active ß-galactosidase from Thalassospira frigidphilosprofundus: a novel marine psychrophile from deep waters of Bay of Bengal.

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Statistical approach for the enhanced production of cold-active ß-galactosidase from Thalassospira frigidphilosprofundus: a novel marine psychrophile from deep waters of Bay of Bengal.

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Statistical approach for the enhanced production of cold-active ß-galactosidase from Thalassospira frigidphilosprofundus: a novel marine psychrophile from deep waters of Bay of Bengal.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Pulicherla, K. K. Kumar, P. Suresh Manideep, K. Rekha, V. P. B. Ghosh, Mrinmoy Sambasiva Rao, K. R. S.
Spatial Coverage	India
Description	Country affiliation: India Author Affiliation: Pulicherla KK ( Center for Bioseparation Technology, VIT University, Vellore, India. pkkanth2006@gmail.com)
Abstract	In the present investigation Thalassospira frigidphilosprofundus, a novel species from the deep waters of the Bay of Bengal, was explored for the production of cold-active ß-galactosidase by submerged fermentation using marine broth medium as the basal medium. Effects of various medium constituents, namely, carbon, nitrogen source, pH, and temperature, were investigated using a conventional one-factor-at-a-time method. It was found that lactose, yeast extract, and bactopeptones are the most influential components for ß-galactosidase production. Under optimal conditions, the production of ß-galactosidase was found to be 3,864 U/mL at 20 ± 2°C, pH 6.5 ± 0.2, after 48 hr of incubation. ß-Galactosidase production was further optimized by the Taguchi orthogonal array design of experiments and the central composite rotatable design (CCRD) of response surface methodology. Under optimal experimental conditions the cold-active ß-galactosidase enzyme production from Thalassospira frigidphilosprofundus was enhanced from 3,864 U/mL to 10,657 U/mL, which is almost three times higher than the cold-active ß-galactosidase production from the well-reported psychrophile Pseudoalteromonas haloplanktis.
File Format	HTM / HTML
ISSN	10826068
Issue Number	8
Volume Number	43
e-ISSN	15322297
Journal	Preparative Biochemistry and Biotechnology
Language	English
Publisher	Taylor & Francis
Publisher Date	2013-01-01
Publisher Place	Great Britain (UK)
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Discipline Biochemistry Discipline Biotechnology Bacterial Proteins Biosynthesis Bays Microbiology Principal Component Analysis Rhodospirillaceae Chemistry Beta-galactosidase Isolation & Purification Carbon Metabolism Cold Temperature Culture Media Fermentation Hydrogen-ion Concentration India Kinetics Lactose Nitrogen Enzymology Journal Article Research Support, Non-u.s. Gov't
Content Type	Text
Resource Type	Article
Subject	Medicine Biochemistry Biotechnology

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