| Author |
Luo, Yu Scholl, Stefan Doering, Anett Zhang, Yi Irani, Niloufer G. Di Rubbo, Simone Neumetzler, Lutz Krishnamoorthy, Praveen Van Houtte, Isabelle Mylle, Evelien Bischoff, Volker Vernhettes, Samantha Winne, Johan Friml, Jirí Stierhof, York-Dieter Schumacher, Karin Persson, Staffan Russinova, Eugenia |
| Description |
Country affiliation: Belgium Author Affiliation: Luo Y ( Department of Plant Systems Biology, VIB, 9052 Gent, Belgium.); Scholl S ( Department of Plant Biotechnology and Bioinformatics, Ghent University, 9052 Gent, Belgium.); Doering A ( Developmental Biology of Plants, Centre for Organismal Studies (COS), Heidelberg University, 69120 Heidelberg, Germany.); Zhang Y ( Max-Planck Institute for Molecular Plant Physiology, 14476 Potsdam, Germany.); Irani NG ( Max-Planck Institute for Molecular Plant Physiology, 14476 Potsdam, Germany.); Di Rubbo S ( Department of Plant Systems Biology, VIB, 9052 Gent, Belgium.); Neumetzler L ( Department of Plant Biotechnology and Bioinformatics, Ghent University, 9052 Gent, Belgium.); Krishnamoorthy P ( Department of Plant Systems Biology, VIB, 9052 Gent, Belgium.); Van Houtte I ( Department of Plant Biotechnology and Bioinformatics, Ghent University, 9052 Gent, Belgium.); Mylle E ( Max-Planck Institute for Molecular Plant Physiology, 14476 Potsdam, Germany.); Bischoff V ( Max-Planck Institute for Molecular Plant Physiology, 14476 Potsdam, Germany.); Vernhettes S ( Department of Plant Systems Biology, VIB, 9052 Gent, Belgium.); Winne J ( Department of Plant Biotechnology and Bioinformatics, Ghent University, 9052 Gent, Belgium.); Friml J ( Department of Plant Systems Biology, VIB, 9052 Gent, Belgium.); Stierhof YD ( Department of Plant Biotechnology and Bioinformatics, Ghent University, 9052 Gent, Belgium.); Schumacher K ( Institut National de la Recherche Agronomique, Unité Mixte de Recherche 1318, Institut Jean-Pierre Bourgin, Saclay Plant Sciences, 78000 Versailles, France.); Persson S ( AgroParisTech, Institut Jean-Pierre Bourgin, 78000 Versailles, France.); Russinova E ( Institut National de la Recherche Agronomique, Unité Mixte de Recherche 1318, Institut Jean-Pierre Bourgin, Saclay Plant Sciences, 78000 Versailles, France.) |
| Abstract |
In plants, vacuolar H(+)-ATPase (V-ATPase) activity acidifies both the trans-Golgi network/early endosome (TGN/EE) and the vacuole. This dual V-ATPase function has impeded our understanding of how the pH homeostasis within the plant TGN/EE controls exo- and endocytosis. Here, we show that the weak V-ATPase mutant deetiolated3 (det3) displayed a pH increase in the TGN/EE, but not in the vacuole, strongly impairing secretion and recycling of the brassinosteroid receptor and the cellulose synthase complexes to the plasma membrane, in contrast to mutants lacking tonoplast-localized V-ATPase activity only. The brassinosteroid insensitivity and the cellulose deficiency defects in det3 were tightly correlated with reduced Golgi and TGN/EE motility. Thus, our results provide strong evidence that acidification of the TGN/EE, but not of the vacuole, is indispensable for functional secretion and recycling in plants. |
