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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Ioannou, D. Meershoek, E. J. Thornhill, A. R. Ellis, M. Griffin, D. K. |
| Description | Country affiliation: United kingdom Author Affiliation: Ioannou D ( School of Biosciences, University of Kent, Giles Lane, CT2 7NJ, Canterbury, UK.) |
| Abstract | From the late 1980s onwards, the use of DNA probes to visualise sequences on individual chromosomes (fluorescent in-situ hybridisation - FISH) revolutionised the study of cytogenetics. Following single colour experiments, more fluorochromes were added, culminating in a 24 colour assay that could distinguish all human chromosomes. Interphase cytogenetics (the detection of chromosome copy number in interphase nuclei) soon followed, however 24 colour experiments are hampered for this application as mixing fluorochromes to produce secondary colours produces images that are not easily distinguishable from overlapping signals. This study reports the development and use of a novel protocol, new fast hybridising FISH probes, and a bespoke image capture system for the assessment of chromosome copy number in interphase nuclei. The multicolour probe sets can be used individually or in sequential hybridisation layers to assess ploidy of all 24 human chromosomes in the same nucleus. Applications of this technique are in the investigation of chromosome copy number and the assessment of nuclear organisation for a range of different cell types including human sperm, cancer cells and preimplantation embryos. |
| File Format | HTM / HTML |
| ISSN | 08908508 |
| Issue Number | 5-6 |
| Volume Number | 25 |
| e-ISSN | 10961194 |
| Journal | Molecular and Cellular Probes |
| Language | English |
| Publisher | Elsevier |
| Publisher Date | 2011-10-01 |
| Publisher Place | Great Britain (UK) |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Discipline Cell Biology Discipline Molecular Biology Discipline Biotechnology Chromosomes, Human Chemistry Cytogenetics Methods Dna Probes Analysis Image Processing, Computer-assisted In Situ Hybridization, Fluorescence Ploidies Blastocyst Cytology Cell Nucleus Genetics Instrumentation Dna Chemical Synthesis Metabolism Fertilization In Vitro Fluorescent Dyes Humans Interphase Male Spermatozoa Tumor Cells, Cultured Journal Article |
| Content Type | Text |
| Resource Type | Article |
| Subject | Cell Biology Molecular Biology |
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