NDLI: Characterization of a Soil Metagenome-Derived Gene Encoding Wax Ester Synthase.

Please wait, while we are loading the content...

Improvement of FK506 Production in the High-Yielding Strain Streptomyces sp. RM7011 by Engineering the Supply of Allylmalonyl-CoA Through a Combination of Genetic and Chemical Approach.

CRISPR/Cas9-Mediated Re-Sensitization of Antibiotic-Resistant Escherichia coli Harboring Extended-Spectrum ß-Lactamases.

Characterization of a Novel Alkaline Family VIII Esterase with S-Enantiomer Preference from a Compost Metagenomic Library.

Investigation of the Properties of Sand Tubules, a Biomineralization Product, and their Microbial Community.

Structural Insight into Dihydrodipicolinate Reductase from Corybebacterium glutamicum for Lysine Biosynthesis.

Purification and Characterization of a New κ-Carrageenase from the Marine Bacterium Vibrio sp. NJ-2.

Process Performance and Bacterial Community Structure Under Increasing Influent Disturbances in a Membrane-Aerated Biofilm Reactor.

Regulation of the Lactobacillus Strains on HMGCoA Reductase Gene Transcription in Human HepG2 Cells via Nuclear Factor-κB.

Physiological Effects of GLT1 Modulation in Saccharomyces cerevisiae Strains Growing on Different Nitrogen Sources.

Characterization of a Soil Metagenome-Derived Gene Encoding Wax Ester Synthase.

Molecular Characterization of Plasmids Encoding CTX-M ß-Lactamases and their Associated Addiction Systems Circulating Among Escherichia coli from Retail Chickens, Chicken Farms, and Slaughterhouses in Korea.

Phage Conversion for ß-Lactam Antibiotic Resistance of Staphylococcus aureus from Foods.

Overproduction of the Escherichia coli Chaperones GroEL-GroES in Rhodococcus ruber Improves the Activity and Stability of Cell Catalysts Harboring a Nitrile Hydratase.

Heterologous Expression and Characterization of a Thermostable Exo-ß-D-Glucosaminidase from Aspergillus oryzae.

Hydrogel Dressing with a Nano-Formula against Methicillin-Resistant Staphylococcus aureus and Pseudomonas aeruginosa Diabetic Foot Bacteria.

Apoptotic Effects of Cordycepin Through the Extrinsic Pathway and p38 MAPK Activation in Human Glioblastoma U87MG Cells.

Cytoprotective Effect of Makgeolli Lees on Paraquat Induced Oxidative Stress in A549 Cells via Activation of NRF2 and Antioxidant Genes.

Surface-Displayed IL-10 by Recombinant Lactobacillus plantarum Reduces Th1 Responses of RAW264.7 Cells Stimulated with Poly(I:C) or LPS.

High-Level Production of Human Papillomavirus (HPV) Type 16 L1 in Escherichia coli.

Isolation and Characterization of Bacteriophages Against Pseudomonas syringae pv. actinidiae Causing Bacterial Canker Disease in Kiwifruit.

Shiga Toxins Trigger the Secretion of Lysyl-tRNA Synthetase to Enhance Proinflammatory Responses.

A Mixture of Ethanol Extracts of Persimmon Leaf and Citrus junos Sieb Improves Blood Coagulation Parameters and Ameliorates Lipid Metabolism Disturbances Caused by Diet-Induced Obesity in C57BL/6J Mice.

Kaempferol Activates G2-Checkpoint of the Cell Cycle Resulting in G2-Arrest and Mitochondria-Dependent Apoptosis in Human Acute Leukemia Jurkat T Cells.

Year: 2016 Volume: 26 Issue: 1

Year: 2015 Volume: 25

Year: 2012 Volume: 22

Year: 2011 Volume: 21

Characterization of a Soil Metagenome-Derived Gene Encoding Wax Ester Synthase.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Kim, Nam Hee Park, Ji-Hye Chung, Eunsook So, Hyun-Ah Lee, Myung Hwan Kim, Jin-Cheol Hwang, Eul Chul Lee, Seon-Woo
Description	Author Affiliation: Kim NH ( Department of Applied Bioscience, Dong-A University, Busan 49315, Republic of Korea.); Park JH ( Department of Applied Bioscience, Dong-A University, Busan 49315, Republic of Korea.); Chung E ( Department of Applied Bioscience, Dong-A University, Busan 49315, Republic of Korea.); So HA ( Department of Applied Bioscience, Dong-A University, Busan 49315, Republic of Korea.); Lee MH ( Department of Applied Bioscience, Dong-A University, Busan 49315, Republic of Korea.); Kim JC ( Department of Agricultural Chemistry, Chonnam National University, Gwangju 61186, Republic of Korea.); Hwang EC ( Department of Applied Bioscience, Dong-A University, Busan 49315, Republic of Korea.); Lee SW ( Department of Applied Bioscience, Dong-A University, Busan 49315, Republic of Korea.)
Abstract	A soil metagenome contains the genomes of all microbes included in a soil sample, including those that cannot be cultured. In this study, soil metagenome libraries were searched for microbial genes exhibiting lipolytic activity and those involved in potential lipid metabolism that could yield valuable products in microorganisms. One of the subclones derived from the original fosmid clone, pELP120, was selected for further analysis. A subclone spanning a 3.3 kb DNA fragment was found to encode for lipase/esterase and contained an additional partial open reading frame encoding a wax ester synthase (WES) motif. Consequently, both pELP120 and the full length of the gene potentially encoding WES were sequenced. To determine if the wes gene encoded a functioning WES protein that produced wax esters, gas chromatography-mass spectroscopy was conducted using ethyl acetate extract from an Escherichia coli strain that expressed the wes gene and was grown with hexadecanol. The ethyl acetate extract from this E. coli strain did indeed produce wax ester compounds of various carbon-chain lengths. DNA sequence analysis of the full-length gene revealed that the gene cluster may be derived from a member of Proteobacteria, whereas the clone does not contain any clear phylogenetic markers. These results suggest that the wes gene discovered in this study encodes a functional protein in E. coli and produces wax esters through a heterologous expression system.
File Format	HTM / HTML
ISSN	10177825
e-ISSN	17388872
Journal	Journal of Microbiology and Biotechnology
Issue Number	2
Volume Number	26
Language	English
Publisher	Korean Society for Microbiology and Biotechnology
Publisher Date	2016-02-01
Publisher Place	Korea (South)
Access Restriction	Open
Subject Keyword	Discipline Microbiology Discipline Biotechnology Acyltransferases Genetics Metabolism Lipase Metagenome Soil Microbiology Chemistry Amino Acid Motifs Amino Acid Sequence Escherichia Coli Enzymology Growth & Development Esterases Genes, Bacterial Genomic Library Lipid Metabolism Phylogeny Sequence Alignment Sequence Analysis, Dna Substrate Specificity Research Support, Non-u.s. Gov't
Content Type	Text
Resource Type	Article
Subject	Medicine Applied Microbiology and Biotechnology Biotechnology

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

A novel cold active esterase derived from Colombian high Andean forest soil metagenome.

Isolation and biochemical characterization of two novel metagenome-derived esterases.

Characterization of a Novel Alkaline Family VIII Esterase with S-Enantiomer Preference from a Compost Metagenomic Library.

Isolation and characterization of a family VII esterase derived from alluvial soil metagenomic library

Identification of lipase encoding genes from Antarctic seawater bacteria using degenerate primers: expression of a cold-active lipase with high specific activity.

Critical residues of class II PHA synthase for expanding the substrate specificity and enhancing the biosynthesis of polyhydroxyalkanoate.

PCR-based amplification and heterologous expression of Pseudomonas alcohol dehydrogenase genes from the soil metagenome for biocatalysis.

Structural and biochemical characterization of a metagenome-derived esterase with a long N-terminal extension.

Screening and identification of a novel esterase EstPE from a metagenomic DNA library.

Characterization of a Soil Metagenome-Derived Gene Encoding Wax Ester Synthase.

Similar Documents

A novel cold active esterase derived from Colombian high Andean forest soil metagenome.

Isolation and biochemical characterization of two novel metagenome-derived esterases.

Characterization of a Novel Alkaline Family VIII Esterase with S-Enantiomer Preference from a Compost Metagenomic Library.

Isolation and characterization of a family VII esterase derived from alluvial soil metagenomic library

Identification of lipase encoding genes from Antarctic seawater bacteria using degenerate primers: expression of a cold-active lipase with high specific activity.

Critical residues of class II PHA synthase for expanding the substrate specificity and enhancing the biosynthesis of polyhydroxyalkanoate.

PCR-based amplification and heterologous expression of Pseudomonas alcohol dehydrogenase genes from the soil metagenome for biocatalysis.

Structural and biochemical characterization of a metagenome-derived esterase with a long N-terminal extension.

Screening and identification of a novel esterase EstPE from a metagenomic DNA library.

Characterization of a Soil Metagenome-Derived Gene Encoding Wax Ester Synthase.