NDLI: A Newly Identified Glutaminase-Free Ê-Asparaginase (Ê-ASPG86) from the Marine Bacterium Mesoflavibacter zeaxanthinifaciens.

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A Newly Identified Glutaminase-Free Ê-Asparaginase (Ê-ASPG86) from the Marine Bacterium Mesoflavibacter zeaxanthinifaciens.

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A Newly Identified Glutaminase-Free Ê-Asparaginase (Ê-ASPG86) from the Marine Bacterium Mesoflavibacter zeaxanthinifaciens.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Lee, Su-Jin Lee, Youngdeuk Park, Gun-Hoo Umasuthan, Navaneethaiyer Heo, Soo-Jin De Zoysa, Mahanama Jung, Won-Kyo Lee, Dae-Won Kim, Hanjun Kang, Do-Hyung Oh, Chulhong
Description	Author Affiliation: Lee SJ ( Jeju International Marine Science Research & Education Center, Korea Institute of Ocean Science & Technology, Jeju Special Self-Governing Province 63349, Republic of Korea.); Lee Y ( Jeju International Marine Science Research & Education Center, Korea Institute of Ocean Science & Technology, Jeju Special Self-Governing Province 63349, Republic of Korea.); Park GH ( Jeju International Marine Science Research & Education Center, Korea Institute of Ocean Science & Technology, Jeju Special Self-Governing Province 63349, Republic of Korea.); Umasuthan N ( Department of Marine Life Sciences, School of Marine Biomedical Sciences, Jeju National University, Jeju Special Self-Governing Province 63243, Republic of Korea.); Heo SJ ( Jeju International Marine Science Research & Education Center, Korea Institute of Ocean Science & Technology, Jeju Special Self-Governing Province 63349, Republic of Korea.); De Zoysa M ( College of Veterinary Medicine, Chungnam National University, Daejeon 34134, Republic of Korea.); Jung WK ( Department of Biomedical Engineering and Centre for Marine-Integrated Biomedical Technology (BK21 Plus), Pukyong National University, Busan 48513, Republic of Korea.); Lee DW ( Marine Ecosystem and Biological Research Center, Korea Institute of Ocean Science & Technology, Ansan 15627, Republic of Korea.); Kim H ( Marine Ecosystem and Biological Research Center, Korea Institute of Ocean Science & Technology, Ansan 15627, Republic of Korea.); Kang DH ( Jeju International Marine Science Research & Education Center, Korea Institute of Ocean Science & Technology, Jeju Special Self-Governing Province 63349, Republic of Korea.); Oh C ( Jeju International Marine Science Research & Education Center, Korea Institute of Ocean Science & Technology, Jeju Special Self-Governing Province 63349, Republic of Korea.)
Abstract	Ê-Asparaginase (E.C. 3.5.1.1) is an enzyme involved in asparagine hydrolysis and has the potential to effect leukemic cells and various other cancer cells. We identified the Lasparaginase gene (Ê-ASPG86) in the genus Mesoflavibacter, which consists of a 1,035 bp open reading frame encoding 344 amino acids. Following phylogenetic analysis, the deduced amino acid sequence of Ê-ASPG86 (Ê-ASPG86) was grouped as a type I asparaginase with respective homologs in Escherichia coli and Yersinia pseudotuberculosis. The Ê-ASPG86 gene was cloned into the pET-16b vector to express the respective protein in E. coli BL21 (DE3) cells. Recombinant Ê-asparaginase (r-Ê-ASPG86) showed optimum conditions at 37-40oC, pH 9. Moreover, r-Ê-ASPG86 did not exhibit glutaminase activity. In the metal ions test, its enzymatic activity was highly improved upon addition of 5 mM manganese (3.97-fold) and magnesium (3.35-fold) compared with the untreated control. The specific activity of r-LASPG86 was 687.1 units/mg under optimum conditions (37°C, pH 9, and 5 mM MnSO4).
File Format	HTM / HTML
ISSN	10177825
e-ISSN	17388872
Journal	Journal of Microbiology and Biotechnology
Issue Number	6
Volume Number	26
Language	English
Publisher	Korean Society for Microbiology and Biotechnology
Publisher Date	2016-06-28
Publisher Place	Korea (South)
Access Restriction	Open
Subject Keyword	Discipline Microbiology Discipline Biotechnology
Content Type	Text
Resource Type	Article
Subject	Medicine Applied Microbiology and Biotechnology Biotechnology

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A Newly Identified Glutaminase-Free Ê-Asparaginase (Ê-ASPG86) from the Marine Bacterium Mesoflavibacter zeaxanthinifaciens.