NDLI: Activator protein-1 mRNA expression, cell proliferation and collagen synthesis induced by tumor necrosis factor-alpha in cardiac fibroblasts from rats and the effect of Qiangxin Decoction

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Activator protein-1 mRNA expression, cell proliferation and collagen synthesis induced by tumor necrosis factor-alpha in cardiac fibroblasts from rats and the effect of Qiangxin Decoction

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Activator protein-1 mRNA expression, cell proliferation and collagen synthesis induced by tumor necrosis factor-alpha in cardiac fibroblasts from rats and the effect of Qiangxin Decoction

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Shen, Yan Yan, Shi-Yun
Description	Country affiliation: China Author Affiliation: Shen Y ( Department of Cardiology, Yueyang Hospital of Integrated Traditional Chinese and Western Medicine, Shanghai University of Traditional Chinese Medicine, Shanghai 200437, China.)
Abstract	OBJECTIVE: To investigate the function of activator protein-1 (AP-1) in proliferation and collagen synthesis of cardiac fibroblasts (CFs) in neonatal rats induced by tumor necrosis factor-alpha (TNF-alpha), and to explore the mechanism of Qiangxin Decoction (QXD), a compound traditional Chinese herbal medicine, in reversing cardiac fibrosis. METHODS: CFs derived from neonatal rats were cultured with enzymatic dissociation, and fibrosis of the CFs was induced by TNF-alpha. The CFs were divided into normal control group, untreated group, 5% QXD-containing serum group, 10% QXD-containing serum group and 20% QXD-containing serum group. After 24-hour culture of QXD-containing serum, AP-1 mRNA was detected by real-time fluorescent quantitative polymerase chain reaction; proliferation and collagen synthesis of CFs were assayed by thiazolyl blue assay (MTT) and measured by hydroxyproline respectively, in order to determine the effect of different dosage decoction on the proliferation and collagen synthesis of CFs. RESULTS: After 24-hour stimulation of TNF-alpha in CFs, compared with those in normal control group, the expression of AP-1 mRNA and cardiac fibroblast proliferation and collagen synthesis increased significantly (P<0.05). However, Qiangxin Decoction could reduce the expression of AP-1 mRNA and decreased the proliferation and collagen synthesis of CFs significantly (P<0.05). CONCLUSION: Qiangxin Decoction can inhibit proliferation and collagen synthesis of CFs induced by TNF-alpha, and reverse cardiac fibrosis, which may be related to its down-regulation of the expression of AP-1.
File Format	HTM / HTML
ISSN	20954964
Journal	Journal of Chinese Integrative Medicine
Issue Number	9
Volume Number	6
Language	Chinese
Publisher	Science Press
Publisher Date	2008-09-01
Publisher Place	China
Access Restriction	Open
Subject Keyword	Discipline Therapeutics Cell Proliferation Drug Effects Collagen Biosynthesis Drugs, Chinese Herbal Pharmacology Fibroblasts Transcription Factor Ap-1 Metabolism Tumor Necrosis Factor-alpha Animals Animals, Newborn Cells, Cultured Cytology Myocardium Rna, Messenger Genetics Random Allocation Rats, Sprague-dawley Research Support, Non-u.s. Gov't
Content Type	Text
Resource Type	Article
Subject	Complementary and Alternative Medicine

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