NDLI: Controlled oxygen reperfusion protects the lung against early ischemia-reperfusion injury in cardiopulmonary bypasses by downregulating high mobility group box 1.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Rong, Jian Ye, Sheng Wu, Zhong-Kai Chen, Guang-Xian Liang, Meng-Ya Liu, Hai Zhang, Jin-Xin Huang, Wei-Ming
Description	Country affiliation: China Author Affiliation: Rong J ( 1Department of Anesthesiology, the First Affiliated Hospital, Sun Yat-sen University, Guangzhou, China.)
Abstract	Restricting oxygen delivery during the reperfusion phase of cardiopulmonary bypass (CPB) protects the heart, but effects on lung ischemia reperfusion (IR) in CPB are unknown. We examined whether extracellular high mobility group box 1 (HMGB1) mediated inflammation during early lung IR injury in CPB. Fourteen healthy canines received CPB with 60 minutes of aortic clamping and cardioplegic arrest, followed by 90 minutes reperfusion. Following surgery, the animals were randomized into control (n = 7) or test (n = 7) groups. Control animals received a constant level of 80% FiO(2) during the entire procedure, and the test group received a gradual increase in FiO(2) during the first 25 minutes of reperfusion. In the test group, the FiO(2) was initiated at 40% and increased by 10% every 5 minutes, to 80%. Histology, lung injury variables, HMGB1 expression, and inflammatory responses were assessed at baseline (T1) and at 25 minutes (T2) and 90 minutes (T3) after starting reperfusion. Treatment with controlled oxygen significantly suppressed lung pathologies, lung injury variables, and inflammatory responses (all P < .001). After lung IR injury, HMGB1 mRNA and protein expressions were significantly decreased in the controlled oxygen group (all P < .001). Controlled oxygen reperfusion is protective in the early stages of lung IR injury in a canine CPB model, and this protection is linked to HMGB1 downregulation.
File Format	HTM / HTML
ISSN	01902148
Issue Number	4
Volume Number	38
e-ISSN	15210499
Journal	Experimental Lung Research
Language	English
Publisher	Taylor & Francis
Publisher Date	2012-05-01
Publisher Place	Great Britain (UK)
Access Restriction	Subscribed
Subject Keyword	Discipline Pulmonary Medicine Acute Lung Injury Prevention & Control Cardiopulmonary Bypass Adverse Effects Hmgb1 Protein Genetics Metabolism Oxygen Administration & Dosage Reperfusion Injury Etiology Animals Base Sequence Cytokines Blood Disease Models, Animal Dogs Down-regulation Drug Effects Female Lung Pathology Male Malondialdehyde Peroxidase Rna, Messenger Journal Article Research Support, Non-u.s. Gov't
Content Type	Text
Resource Type	Article
Subject	Pulmonary and Respiratory Medicine Molecular Biology Clinical Biochemistry

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