NDLI: Detection of peginesatide in equine serum using liquid chromatography-tandem mass spectrometry for doping control purposes.

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Detection of peginesatide in equine serum using liquid chromatography-tandem mass spectrometry for doping control purposes.

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Detection of peginesatide in equine serum using liquid chromatography-tandem mass spectrometry for doping control purposes.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Möller, Ines Thomas, Andreas Wingender, Anke Machnik, Marc Schänzer, Wilhelm Thevis, Mario
Description	Country affiliation: Germany Author Affiliation: Möller I ( Institute of Biochemistry-Center for Preventive Doping Research, German Sport University Cologne, Am Sportpark Müngersdorf 6, 50933 Cologne, Germany.)
Abstract	Erythropoietin (EPO) and its recombinant analogues are suspected to be illicitly administered to horses for performance enhancing purposes and, consequently, prohibited in equine sports. Recently, a new erythropoiesis-stimulating agent, peginesatide (Omontys, formerly referred to as Hematide), belonging to the upcoming class of EPO-mimetic peptides, received approval for the treatment of anaemia in humans with chronic kidney disease on dialysis. As the pegylated dimeric peptide of approximately 45 kDa without sequence homology to EPO is not detectable by conventional EPO detection assays, specific methods are bound to be established for horse sports drug testing. Thus, by fortifying equine serum with peginesatide, an approach consisting of a proteolytic digestion with subtilisin after protein precipitation was developed, eventually targeting a proteotypic and xenobiotic pentapeptide which is easily accessible to liquid chromatography- tandem mass spectrometry analysis. The method was validated for qualitative purposes and demonstrated to be specific, precise (relative standard deviations below 14%), sensitive (limit of detection 10 ng mL(-1)) and linear. Being simple, cost-effective and readily transferable to other doping control laboratories, a mass spectrometric assay for the detection of therapeutic concentrations of peginesatide in equine serum is, in terms of preventive doping research, applicable to routine analysis shortly after approval of the drug.
File Format	HTM / HTML
ISSN	14690667
Issue Number	4
Volume Number	18
e-ISSN	17516838
Journal	European Journal of Mass Spectrometry
Language	English
Publisher	Sage Publications
Publisher Date	2012-01-01
Publisher Place	Great Britain (UK)
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Discipline Analytical Discipline Chemistry Techniques Chromatography, Liquid Methods Doping In Sports Prevention & Control Horses Blood Peptides Substance Abuse Detection Veterinary Tandem Mass Spectrometry Animals Blood Chemical Analysis Journal Article Research Support, Non-u.s. Gov't
Content Type	Text
Resource Type	Article
Subject	Spectroscopy Medicine Atomic and Molecular Physics, and Optics

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