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An ATP Aptasensor Based on the Peroxidase-like Activity of Hemin/Graphene Oxide Nanosheets.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Liang, Ying Su, Jing Huang, Yong Li, Xiaohua Tao, Yiwen Lu, Chaofeng Zhu, Jianmeng Bai, Zhihao Meng, Jinyu Lu, Xiaoling Zhao, Yongxiang
Description	Author Affiliation: Liang Y ( National Center for International Research of Biological Targeting Diagnosis and Therapy, Guangxi Key Laboratory of Biological Targeting Diagnosis and Therapy Research, Collaborative Innovation Center for Targeting Tumor Diagnosis and Therapy, Guangxi Medical University.)
Abstract	In the present work, a sensitive electrochemical aptasensor was designed for the detection of adenosine triphosphate (ATP) with hemin/graphene oxide nanosheets (HGNs). Firstly, the ATP aptamer was self-assembled on gold electrode surface, and then HGNs were captured to the modified electrode by π-π stacking. The captured HGNs could catalyze the disproportionation reaction of H2O2, and produced a detectable electrochemical signal by chronoamperometry. ATP was competitively bound to aptamer which led to the release of HGNs from the electrode surface after adding ATP. The decrease of the electrochemical signal, which was calculated by the difference of amperometric responses before and after incubation of ATP, provided a quantitative signal for ATP detection. A linear correlation was achieved between the difference of the amperometric responses and the logarithmic concentration of ATP ranging from 0.5 to 100 nM with a detection limit of 0.08 nM. Besides, the aptasensor also exhibited good selectivity toward ATP against other analogs.
File Format	HTM / HTML
ISSN	09106340
e-ISSN	13482246
Journal	Analytical Sciences
Issue Number	5
Volume Number	32
Language	English
Publisher	The Japan Society for Analytical Chemistry
Publisher Date	2016-01-01
Publisher Place	Japan
Access Restriction	Open
Subject Keyword	Discipline Analytical Discipline Chemistry Techniques
Content Type	Text
Resource Type	Article
Subject	Analytical Chemistry

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