NDLI: Transmission of porcine circovirus type 2b (PCV2b) in Kunming mice.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Deng, Zhi Bang Yuan, An Wen Luo, Wei Wang, Nai Dong Gong, Qian Long Yu, Xing Long Xue, Li Qun
Description	Author Affiliation: Deng ZB ( Hunan Agricultural University College of Veterinary Medicine Changsha, Hunan 410128 China.)
Abstract	To investigate porcine circovirus type 2b (PCV2b) transmission by contact and vertical infection in Kunming mice (an outbred mouse stock deriving from Swiss albino mice with a high ratio of gene heterozygosis), four mice in cage 6 were inoculated with PCV2b and 25 mice without any treatment were placed into cages 1 to 5 (five mice in each cage). Seven days after being infected, the PCV2-binoculated mice were co-mingled with non-inoculated mice from cages 1 to 5 successively at 7, 14, 21, 28 and 35 days post infection (dpi), respectively, for 3 days. In addition, eleven pregnant mice were injected with PCV2b. Samples were collected from non-inoculated mice and three newborn mice from each litter for PCV2b detection by polymerase chain reaction (PCR) and immunohistochemistry (IHC). The PCR results showed that PCV2b transmission rate among mice in cages 1, 2, 3, 4 and 5 was 0/5, 2/5, 5/5, 5/5 and 1/5, respectively. PCV2b antigen signals generally appeared in most organs of the non-inoculated mice in which viruses were detected by PCR. PCV2b DNA was also detected in newborn mice of PCV2b-infected litters, and viral antigen signals were observed in their organs as well. PCV2b was transmitted in Kunming mice by contact, and it also caused vertical infection through the placenta.
File Format	HTM / HTML
ISSN	02366290
Issue Number	2
Volume Number	61
e-ISSN	02366290
Journal	Acta Veterinaria Hungarica
Language	English
Publisher	Akadémiai Kiadó
Publisher Date	2013-06-01
Publisher Place	Hungary
Access Restriction	Subscribed
Subject Keyword	Discipline Veterinary Medicine Circoviridae Infections Circovirus Animals Antigens, Viral Virology Immunology Dna, Viral Genetics Mice Polymerase Chain Reaction Swine Diseases Journal Article
Content Type	Text
Resource Type	Article
Subject	Veterinary

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