NDLI: First report of molecular identification of Cystoisospora suis in piglets with lethal diarrhea in Japan.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Matsubayashi, Makoto Takayama, Hideko Kusumoto, Masahiro Murata, Misato Uchiyama, Yuka Kaji, Masaya Sasai, Kazumi Yamaguchi, Ryosaku Shibahara, Tomoyuki
Abstract	Cystoisospora suis is a pathogen that causes diarrhea in pigs and can lead to serious disease. Species identification, especially by histopathological examination, is often difficult because of morphologically similar parasites such as Eimeria species. In this study, we used histopathological, bacteriological, virological, and parasitological methods to identify the cause of the disease in two piglets with severe diarrhea. Villous atrophy, diffuse necrosis, and flattening of mucosal epithelial cells were found in the ilea of examined piglets, and coccidian parasites were found in the cytoplasm of the epithelial cells. In some merozoites in the meronts, the presence of two nuclei indicated type 1 merozoites, characteristic of C. suis. According to Cystoisospora-specific PCR targeting the rRNA internal transcribed spacer 1 (ITS1) gene, the sequences of the products were 98.5% similar to those of C. suis. Escherichia coli (O149 serogroup) exhibiting a virulence factor profile (LT, STb, and EAST1 as toxins and F4 as a colonization factor) was detected in one piglet. No other bacteria or significant enteric viruses were found. Co-infection with C. suis and E. coli could imply aggravation of the disease, although further study is needed to assess the pathogenicity of this interaction. This study is the first to clarify by molecular analysis the sequences of C. suis detected in piglets in Japan.
File Format	HTM / HTML
ISSN	12302821
Issue Number	2
Journal	Acta Parasitologica
Volume Number	61
e-ISSN	18961851
Language	English
Publisher	De Gruyter
Publisher Date	2016-03-01
Publisher Place	Poland
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Discipline Parasitology
Content Type	Text
Resource Type	Article
Subject	Parasitology

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