NDLI: Bacterial rapid identification with matrix assisted laser desorption/ionization time-of-flight mass spectrometry: development of an 'in-house method' and comparison with Bruker Sepsityper(®) kit.

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Bacterial rapid identification with matrix assisted laser desorption/ionization time-of-flight mass spectrometry: development of an 'in-house method' and comparison with Bruker Sepsityper(®) kit.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Frédéric Ric, S. Antoine, M. Bodson, A. Lissoir, B.
Description	Country affiliation: Belgium Author Affiliation: Frédéric Ric S ( Trainee in Laboratory Medicine, Université Catholique de Louvain , Belgique.)
Abstract	OBJECTIVE: The objective of this study was to compare an in-house matrix-assisted laser desorption ionization with time of flight (MALDI-TOF) method and a commercial MALDI-TOF kit (Sepsityper(®) kit) for direct bacterial identification in positive blood cultures. We also evaluated the time saved and the cost associated with the rapid identification techniques. METHODS: We used the BACTEC(®) automated system for detecting positive blood cultures. Direct identification using Sepsityper kit and the in-house method were compared with conventional identification by MALDI-TOF using pure bacterial culture on the solid phase. We also evaluated different cut-off scores for rapid bacterial identification. RESULTS: In total, 127 positive blood vials were selected. The rate of rapid identification with the MALDI Sepsityper kit was 25.2% with the standard cut-off and 33.9% with the enlarged cut-off, while the results for the in-house method were 44.1 and 61.4%, respectively. Error rates with the enlarged cut-off were 6.98 (n = 3) and 2.56% (n = 2) for Sepsityper and the in-house method, respectively. Identification rates were higher for gram-negative bacteria. DISCUSSION: Direct bacterial identification succeeded in supplying rapid identification of the causative organism in cases of sepsis. The time taken to obtain a result was nearly 24 âhours shorter for the direct bacterial identification methods than for conventional MALDI-TOF on solid phase culture. Compared with the Sepsityper kit, the in-house method offered better results and fewer errors, was more cost-effective and easier to use.
File Format	HTM / HTML
ISSN	17843286
Issue Number	5
Volume Number	70
e-ISSN	22953337
Journal	Acta Clinica Belgica
Language	English
Publisher	Taylor & Francis
Publisher Date	2015-10-01
Publisher Place	Great Britain (UK)
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Discipline Medicine Bacteremia Microbiology Gram-negative Bacteria Isolation & Purification Gram-positive Bacteria Spectrometry, Mass, Matrix-assisted Laser Desorption-ionization Methods Bacteriological Techniques Humans Comparative Study Journal Article
Content Type	Text
Resource Type	Article
Subject	Medicine

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