NDLI: High-throughput screening compatible cell-based assay for interrogating activated notch signaling.

Please wait, while we are loading the content...

Year: 2017 Volume: 15

Year: 2016 Volume: 14

Year: 2015 Volume: 13

Year: 2014 Volume: 12

Year: 2013 Volume: 11

Year: 2012 Volume: 10

Year: 2011 Volume: 9

Year: 2010 Volume: 8

Year: 2009 Volume: 7

Year: 2009 Volume: 7 Issue: 6

Year: 2009 Volume: 7 Issue: 5

Year: 2009 Volume: 7 Issue: 4

Year: 2009 Volume: 7 Issue: 3

Year: 2009 Volume: 7 Issue: 2

Year: 2009 Volume: 7 Issue: 1

Characterization and optimization of a red-shifted fluorescence polarization ADP detection assay.

WebFlow: a software package for high-throughput analysis of flow cytometry data.

An interview with Garry Nolan, Ph.D. by Glaser Vicki.

The current status of drug discovery against the human kinome.

High-throughput screening compatible cell-based assay for interrogating activated notch signaling.

Bioassays for estrogenic activity: development and validation of estrogen receptor (ERalpha/ERbeta) and breast cancer proliferation bioassays to measure serum estrogenic activity in clinical studies.

AlphaLISA immunoassay platform- the 'no-wash' high-throughput alternative to ELISA.

Year: 2008 Volume: 6

Year: 2007 Volume: 5

High-throughput screening compatible cell-based assay for interrogating activated notch signaling.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Hancock, Michael K. Kopp, Leisha Bi, Kun
Description	Country affiliation: United States Author Affiliation: Hancock MK ( Invitrogen Corporation, Madison, Wisconsin 53719, USA.)
Abstract	Aberrant Notch pathway function is associated with a wide array of developmental disorders, cancers, and neurodegenerative diseases. Thus, strategies to modulate Notch signaling may facilitate therapeutic intervention. Ligand binding to Notch receptors at the cell surface results in a series of cleavage events that release Notch intracellular domain (NICD) fragments that translocate to the nucleus where they function as transcriptional activators of downstream transcriptional programs. We have developed a cell-based assay that can be used to screen for modulators of NICD signaling by engineering HeLa cervical cancer cells with a Notch response element driving beta-lactamase (BLA) reporter gene expression along with a tetracycline-inducible NICD expression system. Induction of NICD expression leads to increased BLA reporter activity that can be knocked down using NICD-specific RNA interference as well as RNA interference against endogenous components of the NICD transactivation complex. Profiling of 19 known compounds in this assay identified several previously undescribed modulators of NICD signaling. The Wnt pathway inhibitor ICG-001 antagonized NICD signaling, whereas the histone deacetylase inhibitor suberoylanilide hydroxamic acid, the heat shock protein 90 inhibitor 17-(allylamino)-17-demethoxygeldanamycin, and the dual phosphatidylinositol 3-kinase (PI3K)/mammalian target of rapamycin inhibitor PI-103 each further activated the NICD-driven reporter activity. The AKT inhibitor triciribine and the PI3K inhibitor GDC-0941 also resulted in the enhanced reporter activity, strongly implicating a role for the PI3K/AKT pathway in regulating NICD signaling. Together this cell-based assay system provides a sensitive, quantitative readout for NICD signaling that is amenable to high-throughput screening for NICD pathway modulators.
File Format	HTM / HTML
ISSN	1540658X
Issue Number	1
Volume Number	7
e-ISSN	15578127
Journal	ASSAY and Drug Development Technologies
Language	English
Publisher	Mary Ann Liebert, Inc.
Publisher Date	2009-02-01
Publisher Place	United States
Access Restriction	Subscribed
Subject Keyword	Discipline Analytical Discipline Pharmacology Discipline Chemistry Techniques Drug Evaluation, Preclinical Methods Receptors, Notch Physiology Signal Transduction Base Sequence Data Interpretation, Statistical Genes, Reporter Genetics Hela Cells Humans Indicators And Reagents Rna Interference Reproducibility Of Results Beta-lactamases Journal Article
Content Type	Text
Resource Type	Article
Subject	Drug Discovery Molecular Medicine

Central Library (ISO-9001:2015 Certified)
Indian Institute of Technology Kharagpur
Kharagpur, West Bengal, India | PIN - 721302

See location in the Map
03222 282435
Mail: support@ndl.gov.in

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in