NDLI: A flexible multiwell format for immunofluorescence screening microscopy of small-molecule inhibitors.

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A flexible multiwell format for immunofluorescence screening microscopy of small-molecule inhibitors.

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A flexible multiwell format for immunofluorescence screening microscopy of small-molecule inhibitors.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Scholz, Anne-Kathrin Klebl, Bert M. Morkel, Markus Lehrach, Hans Dahl, Andreas Lange, Bodo M. H.
Description	Country affiliation: Germany Author Affiliation: Scholz AK ( Department of Vertebrate Genomics, Max-Planck Institute for Molecular Genetics, Berlin, Germany.)
Abstract	Large-scale screens in mammalian cells demand for flexible high-throughput screening platforms that allow to analyze cellular traits on a genome-wide level or to identify small-molecule inhibitors (SMIs) from complex compound libraries. In this study we developed and tested high-density cell arrays made out of polydimethylsiloxane (PDMS) that support cell growth directly on standard glass microscope objective slides. We analyzed the effect of 3 reference inhibitors (MLN-8054, VX-680, and flavopiridol) and 4 exploratory, cell permeable small-molecule kinase inhibitors (two benzothiophene-based and two 4-amino-6-arylpyrimidine-based compounds) on different cell lines, using prototype 5 × 5 and 9 × 9 array carpets. We found that high-density PDMS cell arrays support growth of a broad range of cell types, are well suited for compound screens, and are compatible with high-content screening platforms. This novel array format is of particular advantage for compound screening to identify SMIs, when a combination of flexibility with respect to culture volume, well density, and high-resolution imaging is required. In addition, we demonstrated the suitability of this format for reverse transfection and siRNA experiments.
File Format	HTM / HTML
ISSN	1540658X
Issue Number	5
Volume Number	8
e-ISSN	15578127
Journal	ASSAY and Drug Development Technologies
Language	English
Publisher	Mary Ann Liebert, Inc.
Publisher Date	2010-10-01
Publisher Place	United States
Access Restriction	Subscribed
Subject Keyword	Discipline Analytical Discipline Pharmacology Discipline Chemistry Techniques Antineoplastic Agents Pharmacology Drug Screening Assays, Antitumor High-throughput Screening Assays Protein Kinase Inhibitors Small Molecule Libraries Animals Cos Cells Caco-2 Cells Cell Line, Tumor Cell Proliferation Drug Effects Cell Survival Centrosome Cercopithecus Aethiops Dimethylpolysiloxanes Humans Interphase Microscopy, Fluorescence Mitotic Index Rna, Small Interfering Genetics Tissue Array Analysis Transfection Journal Article Research Support, Non-u.s. Gov't
Content Type	Text
Resource Type	Article
Subject	Drug Discovery Molecular Medicine

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