NDLI: Increased cholesterol 7alpha-hydroxylase expression and size of the bile acid pool in the lactating rat.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Wooton-Kee, Clavia Ruth Cohen, David E. Vore, Mary
Description	Country affiliation: United States Author Affiliation: Wooton-Kee CR ( Graduate Ctr. for Toxicology, University of Kentucky College of Medicine, Lexington, KY 40536, USA.)
Abstract	Maximal bile acid secretory rates and expression of bile acid transporters in liver and ileum are increased in lactation, possibly to facilitate increased enterohepatic recirculation of bile acids. We determined changes in the size and composition of the bile acid pool and key enzymes of the bile acid synthetic pathway [cholesterol 7alpha-hydroxylase (Cyp7a1), sterol 27-hydroxylase (Cyp27a1), and sterol 12alpha-hydroxylase (Cyp8b1)] in lactating rats relative to female virgin controls. The bile acid pool increased 1.9 to 2.5-fold [postpartum (PP) days 10, 14, and 19-23], compared with controls. A 1.5-fold increase in cholic acids and a 14 to 20% decrease in muricholic acids in lactation significantly increased the hydrophobicity index. In contrast, the hepatic concentration of bile acids and small heterodimer partner mRNA were unchanged in lactation. A 2.8-fold increase in Cyp7a1 mRNA expression at 16 h (10 h of light) demonstrated a shift in the diurnal rhythm at day 10 PP; Cyp7a1 protein expression and cholesterol 7alpha-hydroxylase activity were significantly increased at this time and remained elevated at day 14 PP but decreased to control levels by day 21 PP. There was an overall decrease in Cyp27a1 mRNA expression and a 20% decrease in Cyp27a1 protein expression, but there was no change in Cyp8b1 mRNA or protein expression at day 10 PP. The increase in Cyp7a1 expression PP provides a mechanism for the increase in the bile acid pool.
File Format	HTM / HTML
ISSN	01931857
e-ISSN	15221547
DOI	10.1152/ajpgi.00017.2008
Journal	AJP: Gastrointestinal and Liver Physiology
Issue Number	4
Volume Number	294
Language	English
Publisher	American Physiological Society
Publisher Date	2008-04-01
Publisher Place	United States
Access Restriction	Open
Subject Keyword	Discipline Physiology Discipline Gastroenterology Bile Acids And Salts Metabolism Cholesterol 7-alpha-hydroxylase Cholesterol Circadian Rhythm Intestines Lactation Liver Animals Biosynthesis Cholestanetriol 26-monooxygenase Genetics Cholic Acids Enzyme Induction Hydrophobic And Hydrophilic Interactions Enzymology Rna, Messenger Rats, Sprague-dawley Receptors, Cytoplasmic And Nuclear Steroid 12-alpha-hydroxylase Research Support, N.i.h., Extramural
Content Type	Text
Resource Type	Article
Subject	Hepatology Physiology Physiology (medical) Gastroenterology

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