NDLI: In vitro fertilization in inbred BALB/c mice I: isotonic osmolarity and increased calcium-enhanced sperm penetration through the zona pellucida and male pronuclear formation

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Alteration in ultrastructural morphology of bovine embryos following subzonal microinjection of bovine viral diarrhea virus (BVDV).

In vitro fertilization in inbred BALB/c mice I: isotonic osmolarity and increased calcium-enhanced sperm penetration through the zona pellucida and male pronuclear formation

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Year: 2008 Volume: 16 Issue: 1

Year: 2007 Volume: 15

In vitro fertilization in inbred BALB/c mice I: isotonic osmolarity and increased calcium-enhanced sperm penetration through the zona pellucida and male pronuclear formation

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Kito, Seiji Ohta, Yuki
Description	Country affiliation: Japan Author Affiliation: Kito S ( Research Center for Radiation Protection, Department of Advanced Technologies for Radiation Protection Research, National Institute of Radiological Sciences, 4-9-Anagawa, Inage-ku, Chiba 263-8555, Japan. sk126@nirs.go.jp)
Abstract	To optimize IVF conditions for BALB/c mice, which are known to have poor in vitro fertilizability, the requirements for sperm-ova interaction were studied by use of modified simplex optimization medium (mKSOM) as a basic medium. Modified human tubal fluid (mHTF) was used for sperm preincubation and acted as a positive control. When the two media were compared, neither capacitation nor fertilization was supported in mKSOM. Increasing the calcium concentration in mKSOM to 5 mM or more during sperm: ova coincubation improved zona penetration but not male pronuclear (MPN) formation to the same level as those cells incubated in mHTF. When medium osmolarity was varied from 230-305 mOsmol by NaCl at 5 mM CaCl2, MPN formation improved at 280 mOsmol or higher osmolarity to the same level as that found when using mHTF. When NaCl equivalent to 25-75 mOsmol was substituted with trehalose, no significant reduction in fertilization was observed. Substitution of NaCl equivalent to 75 mOsmol with other osmotic reagents (sucrose, choline chloride and sorbitol) resulted in similar levels of fertilization as found with mHTF, except for sorbitol, which reduced fertilization significantly caused by its detrimental effect on sperm viability. At isotonic osmolarity (305 mOsmol), maximum fertilization was observed at 5 mM CaCl2; lower or higher concentrations of CaCl2 resulted in reduced fertilization. Calcium and osmolarity, therefore, are important for sperm : ova interaction in BALB/c mice and the increases in calcium to 5 mM and osmolarity to 305 mOsmol are optimal for BALB/c sperm to penetrate through the zona and to form MPN.
File Format	HTM / HTML
ISSN	09671994
Issue Number	3
Volume Number	16
e-ISSN	14698730
Journal	Zygote
Language	English
Publisher	Cambridge University Press
Publisher Date	2008-08-01
Publisher Place	Great Britain (UK)
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Discipline Embryology Calcium Pharmacology Fertilization In Vitro Sperm-ovum Interactions Drug Effects Zona Pellucida Animals Cell Nucleus Physiology Culture Media Female Isotonic Solutions Male Mice Mice, Inbred Balb C Osmolar Concentration Ovum Cytology Spermatozoa Zygote Journal Article
Content Type	Text
Resource Type	Article
Subject	Cell Biology Developmental Biology

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