NDLI: Effect of trichostatin A on transfected donor cells and subsequent development of porcine cloned embryos.

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Effect of trichostatin A on transfected donor cells and subsequent development of porcine cloned embryos.

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Effect of trichostatin A on transfected donor cells and subsequent development of porcine cloned embryos.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Bo, Fu Di, Liu Qing-chang, Fang Liang, Ren Hong, Ma Liang, Wang Zhen-hua, Guo Zhong-qiu, Li
Description	Author Affiliation: Bo F ( College of Life Science, Northeast Agriculture University, Harbin 150030, PR China.)
Abstract	Transgenes integrated into mammalian cells are silenced rapidly. This phenomenon correlates with repressed chromatin structure marked by histone hypoacetylation. This study investigated the effect of trichostatin A (TSA; a histone-deacetylase inhibitor) on EGFP expression in transfected cells and embryonic development after somatic cell nuclear transfer (SCNT). Porcine adult fibroblasts were transfected with a pEGFP-C1 vector. Then transfected cells, donor cells for SCNT, were pretreated with TSA, with the untreated cells being used as the control. Expression of EGFP in donor cells and reconstructed embryos was detected when exposed to blue light. Results showed that the percentage of EGFP-positive cells significantly increased when the transfected cells were treated with TSA and the increased expression of EGFP was sustained to at least the morula stage. In addition, the cytotoxic effect of TSA on the transfected cells was dose dependent. In conclusion, TSA can rescue the silenced EGFP gene. Even after transferring the TSA-treated cells to enucleated recipient oocytes, TSA retained the ability to rescue a silenced EGFP gene. In addition, TSA had an impact on cell proliferation.
File Format	HTM / HTML
ISSN	09671994
Issue Number	3
Volume Number	19
e-ISSN	14698730
Journal	Zygote
Language	English
Publisher	Cambridge University Press
Publisher Date	2011-08-01
Publisher Place	Great Britain (UK)
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Discipline Embryology Blastocyst Drug Effects Embryo, Mammalian Cytology Embryonic Development Hydroxamic Acids Pharmacology Nuclear Transfer Techniques Oocytes Animals Cell Proliferation Cells, Cultured Fibroblasts Genetic Vectors Green Fluorescent Proteins Genetics Metabolism Histone Deacetylase Inhibitors Swine Transfection Transgenes Journal Article Research Support, Non-u.s. Gov't
Content Type	Text
Resource Type	Article
Subject	Cell Biology Developmental Biology

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