NDLI: A comprehensive method for the conservation of mouse strains combining natural breeding, sperm cryopreservation and assisted reproductive technology.

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Developmental competence of ovine oocyte following vitrification: effect of oocyte developmental stage, cumulus cells, cytoskeleton stabiliser, FBS concentration, and equilibration time.

Cryoprotective effects of low-density lipoproteins, trehalose and soybean lecithin on murine spermatogonial stem cells.

Observation of fresh Bos indicus embryos comparing stereoscopic and phase contrast microscopy.

DNA fragmentation, transgene expression and embryo development after intracytoplasmic injection of DNA-liposome complexes in IVF bovine zygotes.

Distribution of tubulointerstitial nephritis antigen-like 1 and structural matrix proteins in mouse embryos during preimplantation development in vivo and in vitro.

Porcine oocyte vitrification in optimized low toxicity solution with open pulled straws.

Spontaneous generation of reactive oxygen species and effect on motility and fertilizability of sea urchin spermatozoa.

Structural analysis of toad oviductal mucosa in relation to jelly components secretion throughout the reproductive cycle.

DNA methylation pattern in mouse oocytes and their in vitro fertilized early embryos: effect of oocyte vitrification.

Possible regulation of Oct60 transcription by a positive feedback loop in Xenopus oocytes.

Effects of low-density lipoproteins extracted from different avian yolks on boar spermatozoa quality following freezing-thawing.

Replacement of glutamine with the dipeptide derivative alanyl-glutamine enhances in vitro maturation of porcine oocytes and development of embryos.

HSP90 expression correlation with the freezing resistance of bull sperm

Biphasic assembly of the contractile apparatus during the first two cell division cycles in zebrafish embryos.

Characterization of the effects of metformin on porcine oocyte meiosis and on AMP-activated protein kinase activation in oocytes and cumulus cells

Growth factor receptor-bound protein 14: a potential new gene associated with oocyte competence.

Effect of melatonin treatment on developmental potential of somatic cell nuclear-transferred mouse oocytes in vitro.

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A comprehensive method for the conservation of mouse strains combining natural breeding, sperm cryopreservation and assisted reproductive technology.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Li, Dun-Gao Zhu, Yan Li, He-Ping Chen, Xue-Jin Jiang, Man-Xi
Description	Country affiliation: China Author Affiliation: Li DG ( Department of Laboratory Animal Sciences, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China.); Zhu Y ( Key Laboratory of Contraceptive Drugs and Devices of National Population and Family Planning Committee, Shanghai Institute of Planned Parenthood Research, Shanghai 200032, China.); Li HP ( College of Wildlife Resource, Northeast Forestry University, Harbin 150040, China.); Chen XJ ( Department of Laboratory Animal Sciences, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China.); Jiang MX ( Department of Laboratory Animal Sciences, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China.)
Abstract	The maintenance and preservation of strains of mice used in biomedical research presents a unique challenge to individual investigators and research institutions. The goal of this study was to assess a comprehensive system for mouse strain conservation through a combination of natural mating, sperm cryopreservation and assisted reproductive technology. Our strategy was based on the collection and cryopreservation of fresh epididymal sperm from male mice by semi-vasectomy; these mice were then naturally mated for breeding purposes. If no satisfactory results were obtained from natural breeding, then the cryopreserved sperm were used for in vitro fertilization (IVF) and intracytoplasmic sperm injection (ICSI); resultant embryos were then transferred into pseudopregnant-recipient female mice. Our results show that some semi-vasectomized mouse strains can be conserved by natural breeding, and that sterile males can be compensated for through the use of IVF and ICSI technology. As such, we believe this system is suitable for the purpose of strain conservation, allowing the continuation of natural breeding with the safeguard of assisted reproduction available.
File Format	HTM / HTML
ISSN	09671994
Issue Number	2
Volume Number	22
e-ISSN	14698730
Journal	Zygote
Language	English
Publisher	Cambridge University Press
Publisher Date	2014-05-01
Publisher Place	Great Britain (UK)
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Discipline Embryology Breeding Cryopreservation Methods Fertilization In Vitro Spermatozoa Chemistry Animals Cells, Cultured Female Male Mice Mice, Inbred C57bl Mice, Inbred Icr Journal Article Research Support, Non-u.s. Gov't
Content Type	Text
Resource Type	Article
Subject	Cell Biology Developmental Biology

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3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
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7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Effects of preservation of mouse spermatozoa in electrolyte-free solution at 4 degrees C on the outcome of mouse in vitro fertilization.

Osmotic characteristics and fertility of murine spermatozoa collected in different solutions.

Fertilizability, developmental competence, and chromosomal integrity of oocytes microinjected with pre-treated spermatozoa in mice.

Kisspeptin modulates fertilization capacity of mouse spermatozoa.

Comparison of Intrabursal Transfer of Spermatozoa, a New Method for Artificial Insemination in Mice, with Intraoviductal Transfer of Spermatozoa

Pronuclear formation of freeze-dried canine spermatozoa microinjected into mouse oocytes.

In vitro fertilization in inbred BALB/c mice II: effects of lactate, osmolarity and calcium on in vitro capacitation

Fertilization capacity of cryopreserved Iberian ibex epididymal sperm in a heterologous in vitro fertilization assay

Successful in vitro culture of pre-antral follicles derived from vitrified murine ovarian tissue: oocyte maturation, fertilization, and live births.

A comprehensive method for the conservation of mouse strains combining natural breeding, sperm cryopreservation and assisted reproductive technology.

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Effects of preservation of mouse spermatozoa in electrolyte-free solution at 4 degrees C on the outcome of mouse in vitro fertilization.

Osmotic characteristics and fertility of murine spermatozoa collected in different solutions.

Fertilizability, developmental competence, and chromosomal integrity of oocytes microinjected with pre-treated spermatozoa in mice.

Kisspeptin modulates fertilization capacity of mouse spermatozoa.

Comparison of Intrabursal Transfer of Spermatozoa, a New Method for Artificial Insemination in Mice, with Intraoviductal Transfer of Spermatozoa

Pronuclear formation of freeze-dried canine spermatozoa microinjected into mouse oocytes.

In vitro fertilization in inbred BALB/c mice II: effects of lactate, osmolarity and calcium on in vitro capacitation

Fertilization capacity of cryopreserved Iberian ibex epididymal sperm in a heterologous in vitro fertilization assay

Successful in vitro culture of pre-antral follicles derived from vitrified murine ovarian tissue: oocyte maturation, fertilization, and live births.

A comprehensive method for the conservation of mouse strains combining natural breeding, sperm cryopreservation and assisted reproductive technology.