| Content Provider |
World Health Organization (WHO)-Global Index Medicus |
| Author |
Singh, Renu Kumar, Kuldeep Ranjan, R. Kumar, Manish Yasotha, T. Singh, R. K. Das, B. C. Sarkar, M. Bag, Sadhan |
| Description |
Author Affiliation: Singh R ( Indian Veterinary Research Institute,Izatnagar,Bareilly,Uttar Pradesh,India243 122.); Kumar K ( Indian Veterinary Research Institute,Izatnagar,Bareilly,Uttar Pradesh,India243 122.); Ranjan R ( Indian Veterinary Research Institute,Izatnagar,Bareilly,Uttar Pradesh,India243 122.); Kumar M ( Indian Veterinary Research Institute,Izatnagar,Bareilly,Uttar Pradesh,India243 122.); Yasotha T ( Indian Veterinary Research Institute,Izatnagar,Bareilly,Uttar Pradesh,India243 122.); Singh RK ( Indian Veterinary Research Institute,Izatnagar,Bareilly,Uttar Pradesh,India243 122.); Das BC ( Indian Veterinary Research Institute,Izatnagar,Bareilly,Uttar Pradesh,India243 122.); Sarkar M ( Indian Veterinary Research Institute,Izatnagar,Bareilly,Uttar Pradesh,India243 122.); Bag S ( Physiology and Climatology Division,Indian Veterinary Research Institute,Izatnagar-243 122,Bareilly,India.) |
| Abstract |
Aberrant gene expression occurs in parthenogenetic embryos due to abnormal epigenetic modifications in the genome that probably diminish viability and enhance developmental abnormalities in these embryos. In the present study, five developmentally important genes (HPRT1, Cx43, Sox2, Mest and IGF2R) were analysed at different stages in parthenotes (haploid and diploid) and compared with similar stages in in vitro fertilized (IVF) embryos. The results indicated that in haploid parthenotes expression of HPRT1 was upregulated (P < 0.05) only at the 2-4-cell stage whereas Cx43 expression was significantly (P < 0.05) downregulated in all stages as compared with the control. However, expression of this gene was upregulated (P < 0.05) in 2-4-cell and morula stages of diploid parthenotes. Expression of Sox2 was significantly (P < 0.05) downregulated in morula stage haploid parthenotes, whereas it was upregulated (P < 0.05) in 8-16-cell stage diploid embryos. The expression of Mest was upregulated (P < 0.05) at the 2-4-cell stage of both haploid and diploid parthenotes, whereas it was downregulated in 8-16-cell stage diploid embryos as compared with control. IGF2R expression was upregulated (P < 0.05) only in morula stage haploid and diploid parthenote as compared with control. These results indicate that parthenogenetic embryos showed aberrant gene expression of developmentally important genes such as HPRT1, Cx43, Sox2, Mest and IGF2R in comparison with IVF embryos, this finding may be one of the major reasons for the poor developmental competence of parthenogenetic embryos. |
| File Format |
HTM / HTML |
| ISSN |
09671994 |
| Issue Number |
2 |
| Volume Number |
23 |
| e-ISSN |
14698730 |
| Journal |
Zygote |
| Language |
English |
| Publisher |
Cambridge University Press |
| Publisher Date |
2015-04-01 |
| Publisher Place |
Great Britain (UK) |
| Access Restriction |
One Nation One Subscription (ONOS) |
| Subject Keyword |
Discipline Embryology Fertilization In Vitro Gene Expression Regulation, Developmental Goats Embryology Genetics Parthenogenesis Animals Connexin 43 Diploidy Embryo, Mammalian Female Haploidy Hypoxanthine Phosphoribosyltransferase In Vitro Oocyte Maturation Techniques Receptor, Igf Type 2 Soxb1 Transcription Factors Comparative Study Journal Article Research Support, Non-u.s. Gov't |
| Content Type |
Text |
| Resource Type |
Article |
| Subject |
Cell Biology Developmental Biology |
