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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Upadhyay, Santosh Kumar Chandrashekar, Krishnappa |
| Description | Country affiliation: India Author Affiliation: Upadhyay SK ( National Botanical Research Institute, Council of Scientific and Industrial Research, Rana Pratap Marg, Lucknow, 226001 UP, India.) |
| Abstract | Feeding of Helicoverpa armigera larvae on semi-synthetic diet containing Soybean trypsin inhibitor (STI) resulted in disappearance of STI sensitive protease in salivary and midgut protease extract. This might be due to in situ inhibition by dietary STI. STI was largely degraded within 1 h of incubation with total salivary protease (1:1). Degradation was relatively low in midgut proteases. STI interacting proteins were isolated from saliva and midgut extracts of larvae fed on STI supplemented diet using affinity column. Most of the isolated proteins showed caseinolytic activity in zymogram. Denovo sequencing data of seven different peptides selected from trypsin digested total protein showed similarity to chymotrypsinogen, serine protease, aminopeptidase N, peroxidase, hypothetical protein and muscle specific protein. |
| File Format | HTM / HTML |
| ISSN | 15723887 |
| Issue Number | 3 |
| Volume Number | 31 |
| e-ISSN | 15734943 |
| Journal | The Protein Journal |
| Language | English |
| Publisher | Springer |
| Publisher Date | 2012-03-01 |
| Publisher Place | Netherlands |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Discipline Biochemistry Insect Proteins Metabolism Lepidoptera Enzymology Peptide Hydrolases Soybean Proteins Trypsin Inhibitors Amino Acid Sequence Animals Chromatography, Affinity Electrophoresis, Polyacrylamide Gel Gastrointestinal Tract Chemistry Larva Molecular Sequence Data Peptide Fragments Saliva Administration & Dosage Journal Article Research Support, Non-u.s. Gov't |
| Content Type | Text |
| Resource Type | Article |
| Subject | Organic Chemistry Analytical Chemistry Biochemistry Bioengineering |
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